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Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of HEPES and nanobody Nb25

ABSTRACT:

SUBMITTER: Andrija Sente

PROVIDER: EMPIAR-10913 | biostudies-other |

REPOSITORIES: biostudies-other

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Differential assembly diversifies GABAA receptor structures and signalling.

Sente Andrija A Desai Rooma R Naydenova Katerina K Malinauskas Tomas T Jounaidi Youssef Y Miehling Jonas J Zhou Xiaojuan X Masiulis Simonas S Hardwick Steven W SW Chirgadze Dimitri Y DY Miller Keith W KW Aricescu A Radu AR

Nature 20220330 7904

Type A γ-aminobutyric acid receptors (GABAARs) are pentameric ligand-gated chloride channels that mediate fast inhibitory signalling in neural circuits1,2 and can be modulated by essential medicines including general anaesthetics and benzodiazepines3. Human GABAAR subunits are encoded by 19 paralogous genes that can, in theory, give rise to 495,235 receptor types. However, the principles that govern the formation of pentamers, the permutational landsca ...[more]

PMID: 35355020

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