Cryo-EM snapshots of Eukaryotic Membrane Proteins in Native Lipid-Bilayer Nanodiscs
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ABSTRACT:
SUBMITTER: Panagiotis Leonidas Kastritis
PROVIDER: EMPIAR-11252 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-Electron Microscopy Snapshots of Eukaryotic Membrane Proteins in Native Lipid-Bilayer Nanodiscs.
Janson Kevin K Kyrilis Fotis L FL Tüting Christian C Alfes Marie M Das Manabendra M Träger Toni K TK Schmidt Carla C Hamdi Farzad F Vargas Carolyn C Keller Sandro S Meister Annette A Kastritis Panagiotis L PL
Biomacromolecules 20221118 12
New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions. In particular, polymer-encapsulated nanodiscs enable the study of membrane proteins at high resolution while retaining protein-protein and protein-lipid interactions within a lipid bilayer. However, this powerful technology has not been exploited to address the important question of how endogenous─a ...[more]
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