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Ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA

ABSTRACT:

SUBMITTER: Kimon Lemonidis

PROVIDER: EMPIAR-11372 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural and biochemical basis of interdependent FANCI-FANCD2 ubiquitination.

Lemonidis Kimon K Rennie Martin L ML Arkinson Connor C Chaugule Viduth K VK Clarke Mairi M Streetley James J Walden Helen H

The EMBO journal 20221117 3

Di-monoubiquitination of the FANCI-FANCD2 (ID2) complex is a central and crucial step for the repair of DNA interstrand crosslinks via the Fanconi anaemia pathway. While FANCD2 ubiquitination precedes FANCI ubiquitination, FANCD2 is also deubiquitinated at a faster rate than FANCI, which can result in a FANCI-ubiquitinated ID2 complex (IUb D2). Here, we present a 4.1 Å cryo-EM structure of IUb D2 complex bound to double-stranded DNA. We show that this complex, like ID2 ...[more]

PMID: 36385258

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