Dataset Information
SPA cryo-EM micrographs of chicken ubiquitinated FANCD2-FANCI3D with ds 44-bpDNA
Ontology highlight
ABSTRACT:
SUBMITTER: Tamara SIjacki
PROVIDER: EMPIAR-11754 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination.
Nature structural & molecular biology 20220901 9
DNA interstrand cross-links are tumor-inducing lesions that block DNA replication and transcription. When cross-links are detected at stalled replication forks, ATR kinase phosphorylates FANCI, which stimulates monoubiquitination of the FANCD2-FANCI clamp by the Fanconi anemia core complex. Monoubiquitinated FANCD2-FANCI is locked onto DNA and recruits nucleases that mediate DNA repair. However, it remains unclear how phosphorylation activates this pathway. Here, we report structures of FANCD2-F ...[more]