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Cryo-EM of the human CAK: Data for 1.73 Å-resolution reconstruction

ABSTRACT:

SUBMITTER: Basil J Greber

PROVIDER: EMPIAR-11823 | biostudies-other |

REPOSITORIES: biostudies-other

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High-resolution cryo-EM of the human CDK-activating kinase for structure-based drug design.

Cushing Victoria I VI Koh Adrian F AF Feng Junjie J Jurgaityte Kaste K Bondke Alexander A Kroll Sebastian H B SHB Barbazanges Marion M Scheiper Bodo B Bahl Ash K AK Barrett Anthony G M AGM Ali Simak S Kotecha Abhay A Greber Basil J BJ

Nature communications 20240313 1

Rational design of next-generation therapeutics can be facilitated by high-resolution structures of drug targets bound to small-molecule inhibitors. However, application of structure-based methods to macromolecules refractory to crystallization has been hampered by the often-limiting resolution and throughput of cryogenic electron microscopy (cryo-EM). Here, we use high-resolution cryo-EM to determine structures of the CDK-activating kinase, a master regulator of cell growth and division, in its ...[more]

PMID: 38480681

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