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Cytomegalovirus quantitation by real-time PCR is unaffected by delayed separation of plasma from whole blood.


ABSTRACT: A previous study suggested that delayed sample preparation can cause a false-positive plasma cytomegalovirus (CMV) PCR. We evaluated 78 samples submitted for CMV testing, and we report that a 24-h delay in plasma separation did not affect viral quantitation by real-time PCR. Laboratories should evaluate specimen handling requirements for their own PCR technology.

SUBMITTER: Nesbitt SE 

PROVIDER: S-EPMC356862 | biostudies-other | 2004 Mar

REPOSITORIES: biostudies-other

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