Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analysis of lpcat1/2 mutant and wild type plants

ABSTRACT: To explore the transcriptional regulations in lpcat1/2 mutant and wild type plants grown on 1/2 MS media (control), or 1/2 MS media containing 20ug/ml lyso-PAF or 20ug/ml lyso-PAF plus 50 µM ONO

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE128799 | GEO | 2023/03/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

AKGs enriched transcripts associated with PC-, lyso-PC and PAF metabolism

Project description:AKGs enriched transcripts associated with PC-, lyso-PC and PAF metabolism

| PRJNA516229 | ENA

CD4+ T cells gene-transduced with AML1, wild type Foxp3, and a Foxp3 mutant defective in binding to AML1

2007-08-01 | GSE6939 | GEO

Dark grown Arabidopsis seedlings treated with cytokinin

Project description:Arabidopsis seed lings were grown in dark for 10 days on MS media and treated with DMSO (control) or 1 µM of BA for either 45 or 120 minutes to look for changes in genes profiles

2013-03-28 | E-MTAB-1597 | biostudies-arrayexpress

AKGs enriched transcripts associated with PC-, lyso-PC and PAF metabolism

Project description:AKGs enriched transcripts associated with PC-, lyso-PC and PAF metabolism. Moreover, AKGs suppressed interferon response genes. We submit heren three sets of NGS analyses. Experiment 1: We treated mouse adipose tissue macrophages (ATMs) with vehicle (ethanol) or 100 nM alkylglycerols (AKGs) for 18h in vitro. Experiment 2: We treated mouse 3T3-L1 preadipocytes with 1 nM neuropeptide FF (NPFF) for 18 h. Experiment 3: we isolated white adipose tissue from C57Black/6 male mice at 8 weeks of age, and brown adipose tissue from the same mice.

2019-05-14 | GSE125405 | GEO

Transcriptome study of Arabidopsis thaliana plants treated with cellooligosaccharides, cellobiose and control solutions

Project description:Transcriptome analysis was conducted on fourteen-day-old Col-0 Arabidopsis seedlings 1h after treatment with 100 µM cellooligosaccharides, 100 µM cellobiose and control solution. Arabidopsis seedlings were grown on 1/2 MS solid medium in a controlled growth cabinet with a photoperiod of 8 h light (100 µmol photons m-2 s-1) / 16 h darkness, constant temperature of 22 °C. Transcriptome data show that cellooligosaccharides trigger plant immune system

2022-02-01 | E-MTAB-10192 | biostudies-arrayexpress

Transcription profiling of Arabidopsis Col-0 plants with wild type, arr1-1 knock-out, ARR1 over-expressor and ARR1-SRDX fusion protein over-expressor genotypes treated with 6-Benzyladenine for different lengths of time

Project description:Arabidopsis thaliana Col-0 plants and three other genotypes (ARR1 overexpressor, arr1-1 knockout, overexpressor of ARR1-SRDX fusion protein) were grown in liquid media (1/2 MS, 1 g/L sucrose, 0.5 g/L MES, pH 5.7) in a Percival AR-66L growth chamber at 24 oC, 16:8 h day:night cycle, and 100 µE light intensity until growth stage 1.0. Plants were then treated with 5 µM 6-Benzyladenine for 0, 15, and 120 min, harvested and frozen in liquid nitrogen for RNA extraction and subsequent processing for microarray hybridization.

2008-08-31 | E-MEXP-689 | biostudies-arrayexpress

Transcription profiling of mouse CD4+ T cells gene-transduced with AML1, wild type Foxp3, and a Foxp3 mutant defective in binding to AML1

Project description:To clarify how Foxp3 regulates its target genes, we performed co-immunoprecipitation experiments and found that Foxp3 physically bound to AML1/Runx1 (Ono, M. et al, Nature, 2007). In this series of study, we compared gene regulations by AML1, wild type Foxp3, and a Foxp3 mutant with defective binding to AML1. Experiment Overall Design: CD4+ naive T cells were activated and retrovirally gene-transduced with either empty vector (pMCsIg), or AML1-, wild type Foxp3-, or an AML1-non binding mutant Foxp3-encoding vectors. Sixty hours after transfection, 5 x 10^6 GFP-expressing cells were sorted and total RNA was extracted. One cycle target labelling protocol was used for labelling RNA with biotin.

2008-06-15 | E-GEOD-6939 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings supplemented with sucrose, mannitol or atrazine to assess temporal effects of sucrose on atrazine tolerance

Project description:Seeds of Arabidopsis thaliana (ecotype Columbia) were surface-sterilized inbayrochlore/ethanol (1:1, v/v), rinsed in absolute ethanol and dried overnight. Germination and growth were carried out under axenic conditions in square Petri dishes. After seeds were sowed, Petri dishes were placed at 4°C for 48 h in order to break dormancy and homogenize germination, and then transferred to a control growth chamber at 22°C under a 16-h light period regime at 4500 lux for 4 weeks. Growth medium consisted of 0.8% (w/v) agar in 1x Murashige and Skoog (MS) basal salt mix (Sigma, St. Louis, MO, USA) adjusted to pH 5.7. After 4 weeks of cultivation on vertical plates plantlets were transferred on fresh medium complemented with atrazine 10 µM and with sucrose 80mM or mannitol 80 mM which were directly added during preparation of agar-MS media prior to sterilisation. Atrazine was sterilized by microfiltration through 0.2 µm cellulose acetate filters (Polylabo, Strasbourg, France) and then axenically added to melted agar-MS medium prior to pouring into Petri dishes. Then plantlets were harvested after 12, 24 and 48 hours of transfer and extracted for RNA. Plantlets grown on MS medium were harvested and extracted for RNA as control.

2007-03-10 | E-MEXP-574 | biostudies-arrayexpress

Arabidopsis: Control- vs. acetic acid treatment

Project description:Comparing Arabidopsis plants gene expresion in normal conditions (control) with acetic acid treated plants (acetic). Plants were grown on liquid MS media for 13 days, then they were transfered to MS liquid media (control) or MS+3,5 acetic acid (acetic) for two hours.

2011-12-30 | GSE29259 | GEO

Lysophosphatidylcholine acyltransferase 1 controls the mitochondrial reactive oxygen species generation and survival of the retinal photoreceptor cells [P8 photoreceptor]

Project description:Due to the high energy demands and characteristic morphology, retinal photoreceptor cells require a specialized lipid metabolism for survival and function. This study focused on the roles of saturated fatty acids and their metabolism. A frameshift mutation of lysophosphatidylcholine acyltransferase 1 (Lpcat1), introducing saturated fatty acids into lysophosphatidylcholine to produce disaturated phosphatidylcholine (PC), has been reported to cause spontaneous retinal degeneration in mice (rd11 mice).　In this study, we performed a detailed characterization of Lpcat1 in the retina and found that Lpcat1 deficiency induces light-independent and photoreceptor-specific apoptosis in mice. Lipidomic analyses of the retina and isolated photoreceptor outer segment (OS) suggested that loss of Lpcat1 affects disaturated PC production and the proper cellular fatty acid flux, presumably by altering saturated fatty acyl-CoA availabilities. Furthermore, we demonstrated that Lpcat1 deletion increased mitochondrial reactive oxygen species (ROS) levels in photoreceptor cells, but not in other retinal cells without affecting the OS structure and trafficking of OS-localized proteins. These results suggest that the LPCAT1-dependent production of disaturated PC is critical for metabolic adaptation during photoreceptor maturation. Our findings highlight the therapeutic potential of saturated fatty acid metabolism in photoreceptor cell degeneration-related retinal diseases.

2022-01-01 | GSE184817 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data