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Transcriptome analysis of human induced liver cancer-forming cells (iLCCs) , HepG2 cells, Huh6 cells, Huh7 cells, and HuCCT1 cells

ABSTRACT: We found that a set of six transcription factors, FOXA3, HNF1A, HNF1B, LIN28B, L-MYC, and KLF5, can induce direct conversion of human umbilical vein endothelial cells (HUVECs) into liver cancer-forming cells (LCCs). These induced LCCs (iLCCs) efficiently arise tumors that closely resemble human combined hepatocellular cholangiocarcinoma after transplantation. We conducted 3' untranslated region (UTR)-seq (RNA-seq) analyses to investigate the characteristincs of iLCCs, HepG2 cells, Huh6 cells, Huh7 cells, and HuCCT1 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE168997 | GEO | 2022/02/27

REPOSITORIES: GEO

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Transcriptome analysis of liver tumors derived from human induced liver cancer-forming cells (iLCCs)

Project description:We found that a set of six transcription factors, FOXA3, HNF1A, HNF1B, LIN28B, L-MYC, and KLF5, can induce direct conversion of human umbilical vein endothelial cells (HUVECs) into liver cancer-forming cells (LCCs). These induced LCCs (iLCCs) efficiently give rise to tumors that closely resemble human combined hepatocellular cholangiocarcinoma after transplantation to immunodeficient mice. We conducted RNA-seq analyses to investigate the characteristincs of the tumors derived from iLCCs.

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Transcriptome analysis of human induced liver cancer-forming cells (iLCCs) , HepG2 cells, Huh6 cells, Huh7 cells, and HuCCT1 cells

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Project description:Plasticity of differentiated cells has been proved by nuclear transfer, induced pluripotent cells and transdifferentiation. Here we show that by transduction of 3 factors (FOXA3, HNF1A and HNF4A), human fetal fibroblasts can be converted to hepatocyte-like cells (hiHep cells), expressing hepatic marker genes, and acquiring many mature hepatocyte functions in vitro and in vivo. Human fetal fibroblasts (HFF) were tranfected with 3 liver enriched transcription factors (FOXA3, HNF1A, HNF4A), and converted to hepatocyte-like cells (hiHep cells). HFF and primary human hepatocytes (PHH) serve as control.

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