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PSCK9 knockdown in EndoC-bH1 cells time course (DIA secretome time course)

ABSTRACT: EndoC-bH1 cells were incubated with control siRNA or PCSK9 siRNA for 24, 48 and 72 hours. Untreated baseline samples were also included. Unpowered exploratory experiment. Sample size: triplicate per condition.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Type B Pancreatic Cell, Endocrine Pancreas, Cell Culture

SUBMITTER: Maria Ryaboshapkina

LAB HEAD: Raphael Scharfmann

PROVIDER: PXD027920 | Pride | 2022-04-07

REPOSITORIES: Pride

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Characterization of the Secretome, Transcriptome, and Proteome of Human β Cell Line EndoC-βH1.

Ryaboshapkina Maria M Saitoski Kevin K Hamza Ghaith M GM Jarnuczak Andrew F AF Pechberty Séverine S Berthault Claire C Sengupta Kaushik K Underwood Christina Rye CR Andersson Shalini S Scharfmann Raphael R

Molecular & cellular proteomics : MCP 20220402 5

Early diabetes research is hampered by limited availability, variable quality, and instability of human pancreatic islets in culture. Little is known about the human β cell secretome, and recent studies question translatability of rodent β cell secretory profiles. Here, we verify representativeness of EndoC-βH1, one of the most widely used human β cell lines, as a translational human β cell model based on omics and characterize the EndoC-βH1 secretome. We profiled EndoC-βH1 cells using RNA-seq, ...[more]

PMID: 35378291

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PSCK9 knockdown in EndoC-bH1 cells at 72 h (DIA proteomics experiment)

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