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RNA-seq and miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE210056 | GEO | 2023/03/27

REPOSITORIES: GEO

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Gene expression profiling by miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

Project description:In order to define the cohort of miRNAs whose expression is regulated by METTL14 in p53 mutant colorectal cancer, we constructed HT29 cells stably infected with lentivirus carrying control transfectants (vector) or METTL14 overexpression (METTL14). Subsequently, total RNA was isolated and then performed miRNA Microarray.

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RNA-seq and miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

Project description:RNA-seq and miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

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Gene expression profiling by miRNA microarray of control and METTL14-overexpressed HT29 human colon cancer cells

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Project description:This SuperSeries is composed of the SubSeries listed below.

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Diverse effect of WWOX overexpression in HT29 and SW480 colon cancer cell lines.

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-01-23 | E-GEOD-54301 | biostudies-arrayexpress

Gene expression profiling by RNA-seq of control and METTL14-knockdown HT29 human colon cancer cells

Project description:In order to define the cohort of genes whose expression is regulated by METTL14 in p53 mutant colorectal cancer, we constructed HT29 cells stably infected with lentivirus carrying control shRNA (shNC) or METTL14 shRNA (shMETTL14). Subsequently, total RNA was isolated and then performed transcriptome RNA sequence.

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Diverse effect of WWOX overexpression in HT29 and SW480 colon cancer cell lines.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:Soybean isoflavone genistein has multiple anticancer properties and its pro-apoptotic and anti-proliferative effects have been studied in different cancer cells. However, the mechanisms of action of genistein and its molecular targets on human colon cells have not been fully elucidated. Therefore, caspase-3 and p38 mitogen-activated protein kinase (p38 MAPK) as the main therapeutic targets were investigated in this study at both gene expression and protein levels in HT29 colon cancer cells. The caspase-3 and p38 MAPK gene expression levels were examined by real time PCR whereas flow cytometry technique was performed to determine their intracellular protein levels. The caspase-3 enzyme activity was obtained by colorimetric method while the gelatinase activity of matrix metalloproteinase-2 (MMP2) was determined by zymography. In addition, MTT test, wound healing assay and clonogenic assay were carried out to determine the effect of genistein on HT29 cell viability, migration, and proliferation, respectively. Genistein induced apoptotic death in HT29 cells through activation of caspase-3 pathway at the transcriptional, protein, and enzymatic levels. Moreover, genistein inhibited the proliferation of HT29 cells by reducing of both p38 MAPK gene expression and its active phosphorylated protein level. Also, we showed that genistein strongly suppressed the metastatic potency of HT29 colon cancer cells via the reduction of MMP2 activity. Based on the results of this study, we conclude that genistein may exhibit its anticancer properties on HT29 colon cancer cells by modulating caspase-3 and p38 MAPK pathway at different transcriptional and protein levels.

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Diverse effect of WWOX overexpression in HT29 and SW480 colon cancer cell lines.

Project description:WW-domain-containing oxidoreductase (WWOX) is the tumour suppressor gene from the common fragile site FRA16D, whose altered expression has been observed in tumours of various origins. Its suppressive role and influence on basic cellular processes such as proliferation and apoptosis have been confirmed in many in vitro and in vivo studies. Moreover, its protein is thought to take part in the regulation of tissue morphogenesis and cell differentiation. However, its role in colon cancer formation remains unclear. The aim of this study was to characterize the influence of WWOX on the process of colon cancerogenesis, the basic features of the cancer cell and its expression profiles. Multiple biological tests, microarray experiments and quantitative reverse transcriptase (RT)-PCR were performed on two colon cancer cell lines, HT29 and SW480, which differ in morphology, expression of differentiation markers, migratory characteristics and metastasis potential and which represent negative (HT29) and low (SW480) WWOX expression levels. The cell lines were subjected to retroviral transfection, inducting WWOX overexpression. WWOX was found to have diverse effects on proliferation, apoptosis and the adhesion potential of modified cell lines. Our observations suggest that in the HT29 colon cancer cell line, increased expression of WWOX may result in the transition of cancer cells into a more normal colon epithelium phenotype, while in SW480, WWOX demonstrated well-known tumour suppressor properties. Our results also suggest that WWOX does not behave as classical tumour suppressor gene, and its influence on cell functioning is more global and complicated.

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