Influence of human P53 on plant gene expression
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE79678 | GEO | 2016/06/26
SECONDARY ACCESSION(S): PRJNA316694
REPOSITORIES: GEO
Similar Datasets
Project description:Mammalian p53 is a super tumor suppressor and plays a key role in guarding genome from DNA damage. However, p53 has not been found in plants which do not bear cancer although they constantly expose to ionizing radiation of ultraviolet light. Here we introduced p53 into the model plant Arabidopsis and examined p53-conferred phenotype in plant. Most strikingly, p53 caused early senescence and fasciation. We used microarrays to identify differentially expressed genes 10-day-old wild type (Col-0) and 35S:P53-transgenic plants. Replicates are derived from three independent biological experiments.
2016-06-26 | E-GEOD-79678 | biostudies-arrayexpress
Project description:BRCA2A: We used microarrays to identify differentially expressed genes. We focused on those genes that were dramatically salicylic acid-induced (>2-fold) and BRCA2A-dependent in npr1 sni1 double mutants SSN2: We used microarrays to identify differentially expressed genes. We focused on those genes that were dramatically salicylic acid-induced (P<0.05) and SSN2-dependent in npr1 sni1 double mutants
2010-11-21 | GSE23617 | GEO
Project description:Mammalian p53 is a super tumor suppressor and plays a key role in guarding genome from DNA damage. However, p53 has not been found in plants which do not bear cancer although they constantly expose to ionizing radiation of ultraviolet light. Here we introduced p53 into the model plant Arabidopsis and examined p53-conferred phenotype in plant. Most strikingly, p53 caused early senescence and fasciation. In plants, fasciation has been shown as a result of the elevated homologous DNA recombination. Consistently, a reporter with overlapping segments of the GUS gene (1445) showed that the frequency of homologous recombination was highly induced in p53-transgenic plants. In contrast to p53, SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), as a negative regulator of homologous recombination in plants, is not present in mammals. Comet assay and clonogenic survival assay demonstrated that SNI1 inhibited DNA damage repair caused by either ionizing radiation or hydroxyurea in human osteosarcoma U2OS cancer cells. RAD51D is a recombinase in homologous recombination and functions downstream of SNI1 in plants. Interestingly, p53 rendered the sni1 mutants madly branching of inflorescence, a phenotype of fasciation, whereas rad51d mutant fully suppressed the p53-induced phenotype, indicating that human p53 action in plant is mediated by the SNI1-RAD51D signaling pathway. The reciprocal species-swap tests of p53 and SNI1 in human and Arabidopsis manifest that these species-specific proteins play a common role in homologous recombination across kingdoms of animals and plants.
| S-EPMC5029891 | biostudies-literature
Project description:Arabidopsis microarrays identify conserved and differentially-expressed genes involved in shoot grow
| PRJNA93881 | ENA
Project description:We used microarrays to identify genes that are differentially expressed in the absence of miR-998 expression.
2015-08-26 | GSE51724 | GEO
Project description:BRCA2A: We used microarrays to identify differentially expressed genes. We focused on those genes that were dramatically salicylic acid-induced (>2-fold) and BRCA2A-dependent in npr1 sni1 double mutants. SSN2: We used microarrays to identify differentially expressed genes. We focused on those genes that were dramatically salicylic acid-induced (P<0.05) and SSN2-dependent in npr1 sni1 double mutants. BRCA2A: 3-week old npr1 sni1 and npr1 sni1 brca2a mutants were treated with salicylic acid for 16 hours. Replicates are derived from three independent biological experiments. SSN2: 3-week old npr1 sni1 ssn2 mutants were treated with salicylic acid for 16 hours. Replicates are derived from two (SA treated) or three (water treated) independent biological experiments.
2010-11-21 | E-GEOD-23617 | biostudies-arrayexpress
Project description:The vascular tree has considerable diversity, with discrete regions having different physiologic characteristics and permeability. Of note are venules that are significantly more sensitive to pro-inflammatory cytokines than arterioles. We used microarrays to identify molecular signatures that distinguish primary human venous endothelial cells from arterial endothelial cells. We used microarrays to identify genes differentially expressed by venous vs arterial human endothelial cells.
2021-01-18 | GSE137041 | GEO
Project description:Microarrays of gene expression in human germinal center light zone and dark zone B cells sorted according to the expression of cell surface molecules CD83 and CXCR4 We used microarray data to identify genes differentially expressed by B cells in the light and dark zones of germinal centers from human tonsil specimens.
2012-06-14 | GSE38697 | GEO
Project description:We used microarrays to detail the global gene expression and identified differentially expressed gene list between wild-type anterior prostates and Ptenpc-/- anterior prostates, Ptenpc-/-Smad4pc-/- and Ptenpc-/- anterior prostates, Ptenpc-/-p53pc-/- and Ptenpc-/- anterior prostates at 15 weeks of age.
2010-11-05 | GSE25140 | GEO
Project description:In mouse, the adrenocortical dysplasia (acd) phenotype shows limb and body axis anomalies, as a result of p53-dependent apoptosis, and perinatal lethality. The p53 deficiency partially rescues anomalies, but not perinatal lethality, implicating the involvement of p53-independent mechanisms in the acd phenotype. Differentially expressed genes in acd mutant and double mutant embryos were identified. p53-dependent and –independent pathways contributing to acd phenotype were characterized. We used microarrays to analyze gene expression profiles of acd and double mutant embryos and to identify p53 dependent and –independent mechanisms underlying acd phenotype.
2018-09-07 | GSE95806 | GEO