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Cbx3 Maintains Lineage Specificity During Neural Differentiation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE89575 | GEO | 2017/03/27

SECONDARY ACCESSION(S): PRJNA352606

REPOSITORIES: GEO

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Cbx3 maintains lineage specificity during neural differentiation.

Project description:Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage specification by enhancing the expression of neural genes and down-regulating genes specific to alternative fates.

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Cbx3 Maintains Lineage Specificity During Neural Differentiation [RNA-seq]

Project description:Cbx3 (HP1γ) that is a member of the heterochromatin protein 1 family play important roles in development and differentiation. To determine the regulatroy mechanisms of Cbx3 during neural differentiation from ESCs to NPCs, we performed RNA-seq analysis of ESCs or ESC-derived NPCs depleted for Cbx3 or Cbx3-assocatied Mediator subunit Med26.

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Cbx3 Maintains Lineage Specificity During Neural Differentiation [ChIP-seq]

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Cbx3 Maintains Lineage Specificity During Neural Differentiation

Project description:Cbx3 Maintains Lineage Specificity During Neural Differentiation

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