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Metabolomics Workbench ST000680 - GNPS - Functional genomics study on Non Alcoholic fatty liver disease (NAFLD) - part III

ABSTRACT: In this experiment, vector , GCKR (WT), GCKR(P446L), PPP1R3B, TM6SF2 (WT and E167K) overexpression stable HuH-7_Lok cell lines as well as Non-target, GCKR shRNA (52621), PPP1R3B shRNA (2640), TM6SF2 shRNA (382426) knockdown HuH-7_Lok cell lines are used to do the lipidomics experiments 3 X 100 mm dishes of cells were seeded (1x106 cells/dish) for each cell line and incubated at 37? C for 24 hours, then replaced medium with delipidated serum DMEM medium. After 24 hours, treated with BSA-OA (200?M) for another 24 hours before the collection of cells Add 4mL/dish 50 mM ammonium acetate wash cells and then quench cells by adding liquid nitrogen covering all the surface of dish. Store the cell samples in -80?C freezer.

INSTRUMENT(S): QTOF - NEGATIVE, QTOF - POSITIVE

ORGANISM(S): Homo Sapiens

SUBMITTER: Maureen Kachman

PROVIDER: MSV000082706 | GNPS | Thu Aug 02 17:12:00 BST 2018

REPOSITORIES: GNPS

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