Proteomics

Dataset Information

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Phosphoproteomic data of HCT116 cells


ABSTRACT: Quantitative phosphoproteome analyses of colorectal cancer cell lines (HCT116 metastatic clone and the parent clone without metastasis) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS/MS with iTRAQ labeling(metastasis:iTRAQ116 or 117, no metastasis: iTRAQ114 or 115). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Takeshi Tomonaga 

PROVIDER: PXD005174 | JPOST Repository | Tue Oct 18 00:00:00 BST 2016

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
120404_mHCT116_phospho_01.raw Raw
120404_mHCT116_phospho_02.raw Raw
120404_mHCT116_phospho_03.raw Raw
120404_mHCT116_phospho_04.raw Raw
120404_mHCT116_phospho_05.raw Raw
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