Dataset Information

Effect of age on the vascular proteome in middle cerebral arteries and small mesenteric arteries in mice

ABSTRACT: Effect of age on the vascular proteome in middle cerebral arteries and small mesenteric arteries in mice

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Masaki Wakabayashi

PROVIDER: PXD024070 | JPOST Repository | Wed Feb 09 00:00:00 GMT 2022

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	200804_OMCA1_Slot1-01_1_905.d.zip	Other
	200804_OMCA2_Slot1-01_1_906.d.zip	Other
	200804_OMRA1_Slot1-03_1_897.d.zip	Other
	200804_OMRA2_Slot1-03_1_898.d.zip	Other
	200804_YMCA1_Slot1-02_1_901.d.zip	Other

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Publications

Effect of age on the vascular proteome in middle cerebral arteries and mesenteric resistance arteries in mice.

Rabaglino Maria Belen MB Wakabayashi Masaki M Pearson James Todd JT Jensen Lars Jørn LJ

Mechanisms of ageing and development 20211028

Aging is associated with hypertension and brain blood flow dysregulation, which are major risk factors for cardiovascular and neurodegenerative diseases. Structural remodeling, endothelial dysfunction, or hypercontractility of resistance vessels may cause increased total peripheral resistance and hypertension. Recent studies showed that G protein- and RhoA/Rho-kinase pathways are involved in increased mean arterial pressure (MAP) and arterial tone in middle-aged mice. We aimed to characterize th ...[more]

PMID: 34756926

Similar Datasets

Project description:Background: The vascular wall of small arteries is heavily affected by high blood pressure. However, the underlying mechanisms causing vascular changes are not fully elucidated. Using a novel data-independent acquisition mass spectrometry (DIA-MS) approach, we aimed to determine the proteomic changes in small mesenteric arteries during early-onset high blood pressure in a rat model of hypertension. Methods: Snap frozen small mesenteric and renal arteries from the spontaneous hypertension rat (SHR) model and Wistar Kyoto (WKY) control rats were collected from two time points (6- and 12-weels of age) and analyzed by a label free quantitative DIA-MS workflow. Mesenteric arteries from Wister Hannover rats were included as an additional control to clarify genetic drift caused by selective inbreeding. Results: We identified a total of 3956 consistent proteins in the mesenteric artery wall and found that 286 proteins were significantly regulated in 12-weeks old SHRs compared to WKY controls. Comparing to an in silico matrisome database, we identified 38 extracellular matrix-associated proteins that could distinguish SHRs from WKY controls. Furthermore, when comparing the significantly regulated proteins identified in mesenteric and renal arteries, we identified 18 proteins, including Serpina3l, Igg-2a, ENSRNOG00000049829, Acyp2, Enpp3, Lss, Acaa1a, Basp1, an isoform of Basp1, Flot1, Flot2, Gstt1, Nit1, Ppid, Ikbkap, Poglut3, P4ha2 and Usp15, that were changed in both vascular beds. These proteins were associated with vital cellular processes, such as dyslipidemia, protease inhibition, remodeling and generation of reactive oxygen species. Majority of the identified proteins and pathways were associated with hypertension, and mapping the underlying changes help understanding the pathological processes occurring in the arterial wall during early-onset hypertension. Conclusions: Our data provides an in-depth analysis of the proteomic architecture of the mesenteric and renal artery wall from SHRs and WKY control rats. We identified 18 novel candidate proteins that highlights critical changes in small arteries of the SHR.