Dataset Information

Orb-weaver spider (Nephila pilipes) dragline silk proteome

ABSTRACT: Proteome analysis of Nephila pilipes (family Araneidae) dragline silk

ORGANISM(S): Nephila Pilipes

SUBMITTER: Kazuharu Arakawa

PROVIDER: PXD024371 | JPOST Repository | Wed Jul 21 00:00:00 BST 2021

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	170711mm_001_1707_5733_S1_500pmol_001.RAW	Raw
	170711mm_001_1707_5733_S1_500pmol_001.mgf	Mgf
	170711mm_001_1707_5733_S1_500pmol_001.mzML	Mzml
	170711mm_002_1707_5737_S1_500pmol_001.RAW	Raw
	170711mm_002_1707_5737_S1_500pmol_001.mzML	Mzml

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Publications

Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk.

Kono Nobuaki N Nakamura Hiroyuki H Mori Masaru M Yoshida Yuki Y Ohtoshi Rintaro R Malay Ali D AD Pedrazzoli Moran Daniel A DA Tomita Masaru M Numata Keiji K Arakawa Kazuharu K

Proceedings of the National Academy of Sciences of the United States of America 20210801 31

Dragline silk of golden orb-weaver spiders (Nephilinae) is noted for its unsurpassed toughness, combining extraordinary extensibility and tensile strength, suggesting industrial application as a sustainable biopolymer material. To pinpoint the molecular composition of dragline silk and the roles of its constituents in achieving its mechanical properties, we report a multiomics approach, combining high-quality genome sequencing and assembly, silk gland transcriptomics, and dragline silk proteomic ...[more]

PMID: 34312234

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Project description:Spider silk proteins are synthesized in the silk-producing glands, where the spidroins are produced, stored and processed into a solid fiber from a crystalline liquid solution. Despite great interest in the spider silk properties, that make this material suitable for biomedical and biotechnological applications, the mechanism of formation and spinning of the silk fibers has not been fully elucidated; and no combination of proteomic and transcriptomic study has been carried out so far in the spider silk-producing glands. Nephila clavipes is an attractive orb-web spider to investigate the spinning process of silk production, given the properties of strength, elasticity and biocompatibility of their silk fibers. Thus, considering that the combination of proteomic and transcriptomic analysis may reveal an extensive repertoire of novel proteins involved in the silk spinning process, and in order to facilitate and enable proteomics in this non-model organism, the current study aims to construct a high quality reference mRNA-derived protein database that could be used to identify tissue specific expression patterns in spider silk glands. Next-generation sequencing has offered a powerful and cost-efficient technique for the generation of transcriptomic datasets in non-model species using diverse platforms such as the Illumina HiSeq, Roche 454, Pacific Biosystems, and Applied Biosystems SOLiD; In the current study, the Illumina HiSeq 2000 platform will be used to generate a N. clavipes spider silk glands transcriptome-based protein database. The transcriptome data generated in this study will provide a comprehensive and valuable genomic resource for future research of the group of spider silk-producing glands, in order to improve our understanding of the overall mechanism of action involved in production, secretion, storage, transport, protection and conformational changes of spidroins during the spinning process, and prey capture; and the results may be relevant for scientists in material Science, biology, biochemistry, and environmental scientists.