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Orb-weaver spider (Trichonephila clavipes) proteome

ABSTRACT: Proteome analysis of a dragline silk in Trichonephila clavipes (family Araneidae)

ORGANISM(S): Trichonephila Clavipes

SUBMITTER: Kazuharu Arakawa

PROVIDER: PXD024374 | JPOST Repository | Wed Jul 21 00:00:00 BST 2021

REPOSITORIES: jPOST

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			Action	DRS
	170905mm_001_NCV0001_001.RAW	Raw
	170905mm_001_NCV0001_001.mgf	Mgf
	170905mm_001_NCV0001_001.mzML	Mzml
	170905mm_002_NCV0001_002.RAW	Raw
	170905mm_002_NCV0001_002.mgf	Mgf

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Publications

Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk.

Kono Nobuaki N Nakamura Hiroyuki H Mori Masaru M Yoshida Yuki Y Ohtoshi Rintaro R Malay Ali D AD Pedrazzoli Moran Daniel A DA Tomita Masaru M Numata Keiji K Arakawa Kazuharu K

Proceedings of the National Academy of Sciences of the United States of America 20210801 31

Dragline silk of golden orb-weaver spiders (Nephilinae) is noted for its unsurpassed toughness, combining extraordinary extensibility and tensile strength, suggesting industrial application as a sustainable biopolymer material. To pinpoint the molecular composition of dragline silk and the roles of its constituents in achieving its mechanical properties, we report a multiomics approach, combining high-quality genome sequencing and assembly, silk gland transcriptomics, and dragline silk proteomic ...[more]

PMID: 34312234

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Orb-weaver spider (Trichonephila clavata) proteome

Project description:Proteome analysis of a dragline silk in Trichonephila clavata (family Araneidae)

2021-07-21 | PXD024376 | JPOST Repository

Orb-weaver spider (Trichonephila inaurata madagascariensis) dragline silk proteome

Project description:Proteome analysis for a dragline silk in orb-weaver spider (Trichonephila inaurata madagascariensis).

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Orb-weaver spider (Caerostris darwini) proteome

Project description:Proteome analysis of a dragline silk in Darwin's bark spider (Caerostris darwini, family Araneidae)

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Orb-weaver spider (Nephila pilipes) dragline silk proteome

Project description:Proteome analysis of Nephila pilipes (family Araneidae) dragline silk

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An RNA Virome Associated to the Golden Orb-Weaver Spider Nephila clavipes.

Project description:The golden orb-weaver spider Nephila clavipes, known for its sexual size dimorphism, is abundant and widespread in the New World. The first annotated genome of orb-weaver spiders, exploring N. clavipes, has recently been reported. The study, focused primarily on the diversity of silk specific genes, shed light into the complex evolutionary history of spiders. Furthermore, a robust transcriptome analysis provided a massive resource for N. clavipes RNA survey. Here, I present evidence of viral sequences corresponding to the first 10 extant virus species associated to N. clavipes and indeed, nephilids. The putatively new species are linked to ssRNA positive-strand viruses, such as Picornavirales, and to ssRNA negative-strand and dsRNA viruses. In addition, I detected sequence data of new strains of two recently reported arthropod viruses, which complemented and extended the corresponding sequence references. The identified viruses appear to be complete, potentially functional, and presenting the typical architecture and consistent viral domains. The intrinsic nature of the detected sequences and their absence in the recently generated genome assembly, suggest that they correspond to bona fide RNA virus sequences. The available RNA data allowed for the first time to address a tissue/organ specific analysis of virus loads/presence in spiders, suggesting a complex spatial and differential distribution of the tentative viruses, encompassing the spider brain and also silk and venom glands. Until recently, the virus landscape associated to spiders remained elusive. The discovered viruses described here provide only a fragmented glimpse of the potential magnitude of the Aranea virosphere. Future studies should focus not only on complementing and expanding these findings, but also on addressing the potential ecological role of these viruses, which might influence the biology of these outstanding arthropod species.

| S-EPMC5660997 | biostudies-literature

Proximate mechanism of behavioral manipulation of an orb-weaver spider host by a parasitoid wasp.

Project description:Some ichneumonid wasps induce modifications in the web building behavior of their spider hosts to produce resistant "cocoon" webs. These structures hold and protect the wasp's cocoon during pupa development. The mechanism responsible for host manipulation probably involves the inoculation of psychotropic chemicals by the parasitoid larva during a specific developmental period. Recent studies indicate that some spiders build cocoon webs similar to those normally built immediately before ecdysis, suggesting that this substance might be a molting hormone or a precursor chemical of this hormone. Here, we report that Cyclosa spider species exhibiting modified behavior presented higher 20-OH-ecdysone levels than parasitized spiders acting normally or unparasitized individuals. We suggest that the lack of control that spiders have when constructing modified webs can be triggered by anachronic activation of ecdysis.

| S-EPMC5291528 | biostudies-literature

The complete mitochondrial genome of an orb-weaver spider <i>Araneus angulatus</i> (Araneae: Araneidae).

Project description:The complete mitochondrial genome of an orb-weaver spider Araneus angulatus is 14,205 bp in length and contains 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and a control region. The A + T content of the major strand is 75.4%. Nine of the protein-coding genes are initiated by typical ATN codons, one gene (COI) starts with TTA and other three genes (ND6, COII, and COIII) begin with TTG. The truncated stop codon (T) occurs in ND3, ND4L, and ND6, whereas the rest ten genes end with the canonical stop codon (TAA and TAG). Fifteen tRNAs lack the potential to form the typically cloverleaf-shaped secondary structure. The control region is 649 bp in length and contains a long tandem repeat region. The result of phylogenetic analysis shows that the relationship of A. angulatus was close to the species in the same family Araneidae.

| S-EPMC7707736 | biostudies-literature

Detect the shared protein components between spider dragline silk and silkworm cocoon by Label free quantitative proteomics

Project description:The spider major ampullate gland and silkworm silk gland have similar morphological structure and function, so we explored whether there are some common protein components on silks, with three biological replicates for each sample. Dragline silk samples from mature female Trichonephila clavata spider and cocoon samples are from silkworm Bombyx mori chain. The dragline silk of the spider T. clavata and cocoon silk of the silkworm B. mori were cut with scissors. First, silk samples (20 mg of each sample) were dissolved in 500 μL of 9 M LiSCN, and the supernatant was collected after centrifugation (10,000 × g, 10 min). Protein concentrations were measured via the Bradford protein assay (Beyotime, China). Then, the samples were diluted 10-fold in an 8 M urea solution, mixed with 5 × SDS–PAGE buffer, and separated using a NuPAGE 4–12% Bis-Tris protein gel (Thermo Fisher Scientific, USA). The samples were subsequently digested using trypsin, and peptides were measured via LC–MS/MS on a Q Exactive HF-X column.

2022-12-09 | PXD038734 |

Age variation in the body coloration of the orb-weaver spider Alpaida tuonabo and its implications on foraging.

Project description:Spiders show a repertoire of strategies to increase their foraging success. In particular, some orb-weaver spiders use attractive body colorations to lure prey. Interestingly, coloration varies with age in many species, which may result in ontogenetic variation of foraging success. By using field observations, laboratory experiments and spectrophotometric analysis, we investigated whether pale juveniles and bright adults of the orb-weaver Alpaida tuonabo use different foraging strategies due to ontogenetic variation in coloration. Field observations revealed that foraging success of juveniles and adults was influenced by web properties. However, foraging success increased with body size only in adults, supporting the idea that larger individuals produce a stronger visual signal for prey. The attractiveness of the adult coloration for prey was confirmed in the laboratory with frame-web-choice experiments, in which webs bearing a spider intercepted more bees than empty webs. Our spectrophotometric analysis suggests that the yellow coloration may produce the deceiving signal for prey. Moreover, we identified potential alternative foraging strategies: cryptic juveniles at higher heights and 'attractive' adults at lower heights. This study reveals how ontogenetic colour variation may favour the use of alternative foraging strategies in orb-weaver spiders and reduces intraspecific competition.

| S-EPMC5827658 | biostudies-literature

Argiope (Orb weaver) aggregate glue protein composition

Project description:Using proteomics, we documented the glue composition in two congeners that live in different environments, Argiope argentata (dry southwest US) and A. trifasciata (humid southeast US). The viscoelastic protein cores of A. argentata droplets comprised a smaller portion of droplet volume than did those of A. trifasciata and, as humidity increased, incorporated a smaller percentage of absorbed water. Argiope argentata core protein was many times stiffer and tougher than A. trifasciata protein. Each species’ glue included ~30 aggregate-expressed proteins, most of which (24 and 23, respectively) were homologous between the two species. However, the relative contribution and number of gene family members of each homologous group differed. For instance, the aggregate spidroins (AgSp1 and AgSp2) accounted for nearly half of the detected glue composition in A. argentata, but only 38% in A. trifasciata. Additionally, AgSp1, which has highly negatively charged regions, was ~2X as abundant as the positively charged AgSp2 in A. argentata but ~3X as abundant in A. trifasciata. As another example, A. argentata glue included 11 members of a newly discovered cysteine-rich gene family, versus 5 in A. trifasciata. The ability to selectively express different glue protein genes and/or to extrude their products at different rates provides a faster mechanism to evolve material properties than sequence evolution alone.

2023-07-13 | PXD037612 | Pride

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