Proteomics

Dataset Information

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Analysis of Mettl1 interacting proteins in Drosophila

ABSTRACT: Mass spec analysis was performed to identify interacting proteins with FLAG-tagged Mettl1 in Drosophila ovary.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Kuniaki Saito 

PROVIDER: PXD044501 | JPOST Repository | Tue Aug 20 00:00:00 BST 2024

REPOSITORIES: jPOST

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Dataset's files

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Action DRS
Result_a_1_MS16_HEPES-NP40.xlsx Xlsx
Result_a_2_MS16_Tris-Triton.xlsx Xlsx
Result_b_1_MS17_HEPES-NP40.xlsx Xlsx
Result_b_2_MS17_Tris-Triton.xlsx Xlsx
a_1_1_rep1_MS16_HEPES-NP40_anti-FLAG_M2.raw Raw
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Publications

Mettl1-dependent m<sup>7</sup>G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster.

Kaneko Shunya S   Miyoshi Keita K   Tomuro Kotaro K   Terauchi Makoto M   Tanaka Ryoya R   Kondo Shu S   Tani Naoki N   Ishiguro Kei-Ichiro KI   Toyoda Atsushi A   Kamikouchi Azusa A   Noguchi Hideki H   Iwasaki Shintaro S   Saito Kuniaki K  

Nature communications 20240924 1

Modification of guanosine to N<sup>7</sup>-methylguanosine (m<sup>7</sup>G) in the variable loop region of tRNA is catalyzed by the METTL1/WDR4 heterodimer and stabilizes target tRNA. Here, we reveal essential functions of Mettl1 in Drosophila fertility. Knockout of Mettl1 (Mettl1-KO) causes no major effect on the development of non-gonadal tissues, but abolishes the production of elongated spermatids and mature sperm, which is fully rescued by expression of a Mettl1-transgene, but not a catalyt  ...[more]

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