Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tao et al. BioID of Irx3 and Irx5

ABSTRACT: This submission contains the mass spectrometry files for the manuscript by Tao et al. that describes the characterization of the two transcription factors Irx3 and Irx5 and their role in regulating sister chromatid segregation. This submission contains 8 DDA files for the following baits: BirA*-FLAG-Irx3, BirA*-FLAG-Irx5, BirA*-FLAG-GFP (control) and BirA* (control).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras

PROVIDER: MSV000079812 | MassIVE | Sun Jun 12 11:10:00 BST 2016

SECONDARY ACCESSION(S): PXD004309

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Uuskula_P112_VS2_TOP2B_BioID_15JUN2015

Project description:This submission contains the mass spectrometry files for the manuscript by Uuskula-Reimand et al. that investigates the interactome of TOP2B using BioID and its genomic binding profile. This submission contains 18 IDA files for the following baits: empty 3XFLAG control, GFP-BirA-FLAG control, NLS-BirA-FLAG control, TOP2B-BirA-FLAG. See the README file within "Methods and Protocols" and the accompanying File description.

2015-07-10 | MSV000079188 | MassIVE

Lambert_P82_VS4_Chromatin_BioID_APMS_12JUL2014

Project description:This submission contains the mass spectrometry files for the manuscript by Lambert et al. that describes the use of BioID for chromatin associated protein complexes. A detail comparison to a chromatin optimized AP-MS protocol was also performed. This submission contains 39 IDA files for the following baits: empty 3XFLAG control, GFP-BirA-FLAG control, NLS-BirA-FLAG control, H2B-BirA-FLAG, H3.1-BirA-FLAG, 3XFLAG-MED4, BirA-FLAG-MED4, MED20-3XFLAG, MED20-BirA-FLAG, 3XFLAG-MED23 and BirA-FLAG-MED23. Two biological replicates for every bait were analyzed by the standard Gingras lab BioID protocol and by a protocol optimized for chromatin associated baits (AP-MS). See the README file within "Methods and Protocols" and the accompanying File description. Additional details and files are found on prohits-web.lunenfeld.ca.

2014-07-17 | MSV000078782 | MassIVE

Lambert_P82_VS6_ZMYND8_reader_APMS_BioID_01OCT2015

Project description:This submission contains the mass spectrometry files for the manuscript by Filippakopoulos and colleges that characterize ZMYND8 by AP-MS and BioID. This submission contains 56 DDA files for the following baits: empty 3XFLAG control, GFP-BirA-FLAG control, NLS-BirA-FLAG control, 3xFLAG-ZMYND8 and 3xFLAG-ZMYND8 N288F. Two biological replicates for every baits were analyzed by the standard Gingras lab BioID protocol and by a protocol optimized for chromatin associated baits (AP-MS). See the README file within "Methods and Protocols" and the accompanying File description. This dataset consists of 56 raw MS files, all acquired on a 5600 TripleTOF Raw files are deposited here in MassIVE. For questions, please contact Anne-Claude Gingras (gingras@lunenfeld.ca) or Jean-Philippe Lambert (lambert@lunenfeld.ca). Internal reference from the Gingras lab ProHits implementation: Project 82, Export versions VS6 (ZMYND8_reader)

2015-10-01 | MSV000079336 | MassIVE

P30_VS1_Campbell_RNF146_Feb2016

Project description:This submission contains the mass spectrometry files for the manuscript by Campbell et al. that describes the characterization of a protein complex containing RNF146 and its respective mutants involved in epithelial polarity. This submission contains 12 DDA files for the following baits: empty 3XFLAG control, GFP-BirA control, RNF146 WT-BirA, and mutants H53A, R163A, and the double mutant H53A_R163A.

2016-02-04 | MSV000079493 | MassIVE

BioID analysis of human protein GNL3

Project description:We performed a BioID experiment in a HEK293 Flp-In T-Rex cell line that contains human GNL3 cDNA integrated at the FRT site tagged with BirA and FLAG under the control of a promoter regulated with doxycycline.

2023-12-05 | PXD045389 | Pride

BCL11A cistrome in mouse erythroleukemia (MEL) cells

Project description:ChIP-seq analyses were performed in MEL cells expressing BirA alone or BirA and FLAG-Biotin tagged BCL11A (XL isoform).

2014-04-01 | GSE28334 | GEO

BioID MS data for Gfi1b regulates the level of Wnt Beta-catenin signaling in hematopoietic stem cells and megakaryocytes, Nat Com 2018

Project description:4274_OF_20170925_MOR_04: BirA*-Flag (control) replicate 1 4277_OF_20170925_MOR_01: GFI1B-BirA*-Flag replicate 1 4279_OF_20170925_MOR_03_new: BirA*-Flag (control) replicate 2 4286_OF_20170925_MOR_02_new: GFI1B-BirA*-Flag replicate 2 OF_20171106_MOR_01_new: Betacatenin-BirA*-Flag transfected with Empty Vector, replicate 1 OF_20171106_MOR_02_new: Betacatenin-BirA*-Flag transfected with Empty Vector, replicate 2 OF_20171106_MOR_03_new: Betacatenin-BirA*-Flag transfected with GFI1B, replicate 1 OF_20171106_MOR_04_new: Betacatenin-BirA*-Flag transfected with GFI1B, replicate 2 OF_20171107_MOR_01_new: Betacatenin-BirA*-Flag transfected with GFI1B DeltaSnag, replicate 1 OF_20171107_MOR_02_new: Betacatenin-BirA*-Flag transfected with GFI1B DeltaSnag, replicate 2 OF_20171107_MOR_03_new: Betacatenin-BirA*-Flag transfected with GFI1B DeltaWRD, replicate 1 OF_20171107_MOR_04_2_new: Betacatenin-BirA*-Flag transfected with GFI1B DeltaWRD, replicate 2 VL_20120305_MOR_01_new: GFI1B Flag AP-MS data VL_20120305_MOR_02_new: GFI1B Flag AP-MS data VL_20120305_MOR_03_new: GFI1B Flag AP-MS data VL_20120305_MOR_04_new: GFI1B Flag AP-MS data VL_20120305_MOR_05_new: GFI1B Flag AP-MS data VL_20120305_MOR_06_new: GFI1B Flag AP-MS data VL_20120305_MOR_07_new: GFI1B Flag AP-MS data VL_20120305_MOR_08_new: GFI1B Flag AP-MS data

2018-11-09 | MSV000083125 | MassIVE

Feng_P120_VS1_BRPF3_HBO1_complex

Project description:This submission contains the mass spectrometry files for the manuscript by Feng et al. that describes the characterization of a protein complex containing BRPF3 and HBO1 in the regulation of DNA replication origins. This submission contains 3 IDA files for the following baits: empty 3XFLAG control, BRPF1-3XFLAG-HA and BRPF3-3XFLAG-HA.

2014-11-19 | MSV000078943 | MassIVE

BCL11A cistrome in mouse erythroleukemia (MEL) cells

Project description:ChIP-seq analyses were performed in MEL cells expressing BirA alone or BirA and FLAG-Biotin tagged BCL11A (XL isoform). BCL11A chromatin occupancy in MEL cell line.

2014-04-01 | E-GEOD-28334 | biostudies-arrayexpress

Asymmetric self-renewal associated (ASRA) genes

Project description:Cell lines geneticially engineered to undergo conditional asymmetric self-renewal were used to identify genes whose expression is asymmetric self-renewal associated (ASRA). Non-random sister chromatid segregation occurs concordantly with asymmetric self-renewal in these cell lines. Asymmetric self-renewal occurs when murine embryo fibroblasts that are otherwise p53-null are induced to express physiological levels of wildtype p53 protein (Asym). To distinguish p53-responsive genes that also require induction of asymmetric self renewal (i.e., ASRA genes) and/or non-random sister chromatid segregation for change, an additional control cell line, which continues to symmetrically self-renew (with random sister chromatid segregation) even when p53 is induced, was also compared (Symp53). This congenic cell line constitutively expresses the type II inosine monophosphate dehydrogenase (IMPDH II; the rate-limiting enzmye for guanine ribonucleotide biosynthesis) and, thereby, prevents p53-induced asymmetric self-renewal and non-random sister chromatid segregation.

2010-12-22 | GSE25334 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data