Dataset Information

Franco-Swiss HPP project (Chromosomes 2 and 14)

ABSTRACT: The dataset is a concatenation of nanoLC-MS/MS analyses performed on different sample types/sample preparations of human proteins on various instrumental platforms in the laboratory. A franco-swiss collaboration to identify missing human proteins on chromosomes 2 and 14 in the frame of the Human Proteome Project, set up a bioinformatic screen over all datasets accumulated in the laboratory in order to detect proteins classified as “missing” by NextProt.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Odile Bruley Schiltz

PROVIDER: MSV000080273 | MassIVE | Fri Oct 21 21:00:00 BST 2016

SECONDARY ACCESSION(S): PXD002131

REPOSITORIES: MassIVE

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Publications

Computational and Mass-Spectrometry-Based Workflow for the Discovery and Validation of Missing Human Proteins: Application to Chromosomes 2 and 14.

Carapito Christine C Lane Lydie L Benama Mohamed M Opsomer Alisson A Mouton-Barbosa Emmanuelle E Garrigues Luc L Gonzalez de Peredo Anne A Burel Alexandre A Bruley Christophe C Gateau Alain A Bouyssié David D Jaquinod Michel M Cianferani Sarah S Burlet-Schiltz Odile O Van Dorsselaer Alain A Garin Jérôme J Vandenbrouck Yves Y

Journal of proteome research 20150722 9

In the framework of the C-HPP, our Franco-Swiss consortium has adopted chromosomes 2 and 14, coding for a total of 382 missing proteins (proteins for which evidence is lacking at protein level). Over the last 4 years, the French proteomics infrastructure has collected high-quality data sets from 40 human samples, including a series of rarely studied cell lines, tissue types, and sample preparations. Here we described a step-by-step strategy based on the use of bioinformatics screening and subseq ...[more]

PMID: 26132440

Dataset Information

Franco-Swiss HPP project (Chromosomes 2 and 14)

Dataset's files

Publications

Computational and Mass-Spectrometry-Based Workflow for the Discovery and Validation of Missing Human Proteins: Application to Chromosomes 2 and 14.

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