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Pancreatic beta cell proteome with increased proliferation in NOD mice

ABSTRACT: We investigated the pancreatic beta cell proteome changes in NOD-LIRKO vesus NOD where beta cell mass was enhanced early in life. Proteomics data is derived from FACS-sorted beta cells at 8 weeks and 12 weeks.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Wei-Jun Qian

PROVIDER: MSV000083576 | MassIVE | Thu Mar 14 15:31:00 GMT 2019

SECONDARY ACCESSION(S): PXD013100

REPOSITORIES: MassIVE

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Increased β-cell proliferation before immune cell invasion prevents progression of type 1 diabetes.

Dirice Ercument E Kahraman Sevim S De Jesus Dario F DF El Ouaamari Abdelfattah A Basile Giorgio G Baker Rocky L RL Yigit Burcu B Piehowski Paul D PD Kim Mi-Jeong MJ Dwyer Alexander J AJ Ng Raymond W S RWS Schuster Cornelia C Vethe Heidrun H Martinov Tijana T Ishikawa Yuki Y Teo Adrian Kee Keong AKK Smith Richard D RD Hu Jiang J Haskins Kathryn K Serwold Thomas T Qian Wei-Jun WJ Fife Brian T BT Kissler Stephan S Kulkarni Rohit N RN

Nature metabolism 20190506 5

Type 1 diabetes (T1D) is characterized by pancreatic islet infiltration by autoreactive immune cells and a near-total loss of β-cells<sup>1</sup>. Restoration of insulin-producing β-cells coupled with immunomodulation to suppress the autoimmune attack has emerged as a potential approach to counter T1D<sup>2-4</sup>. Here we report that enhancing β-cell mass early in life, in two models of female NOD mice, results in immunomodulation of T-cells, reduced islet infiltration and lower β-cell apoptos ...[more]

PMID: 31423480

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Transcriptional profiling of nascent and mature pancreatic beta cells

Project description:This experiment used RNA-Seq technology to examine transcription profile in FACS-sorted MIP-EGFP+ mouse pancreatic cells at E16.5 (nascent beta cells) and P60 (mature beta cells). Such an analysis should reveal the gene transcription alterations for beta cell development and for function.

2015-10-01 | E-MTAB-3351 | biostudies-arrayexpress

Increased β-cell proliferation prior to immune-cell invasion prevents progression of type 1 diabetes

Project description:Type 1 diabetes (T1D) is characterized by pancreatic islet infiltration by autoreactive immune cells and a near-total loss of β-cells. Restoration of insulin-producing β-cells coupled with immunomodulation to suppress the autoimmune attack has emerged as a potential approach to counter T1D. Here we report that enhancing β-cell mass in female NOD mice early in life (prior to weaning) results in immunomodulation of T-cells, reduced islet infiltration and lower β-cell apoptosis, that together protect them from developing T1D. We observed that a model exhibiting β-cell hyperplasia on the NOD background (NOD-LIRKO) displayed altered β-cell antigens, and islet transplantation studies showed prolonged graft survival of NOD-LIRKO islets even upon exposure to diabetogenic splenocytes in vivo. Adoptive transfer of splenocytes from the NOD-LIRKOs prevented diabetes development in pre-diabetic NOD mice, while conversely, similar protective outcomes were obtained when NOD-LIRKO splenocytes were adoptively transferred after mixing them with diabetogenic NOD splenocytes in a dose-dependent manner. A significant increase in the splenic CD4+CD25+FoxP3+ regulatory T-cell (Treg) population in the NOD-LIRKO mice was observed to drive the protected phenotype since Treg depletion rendered NOD-LIRKO mice diabetic. The increase in Tregs coupled with a downregulation of key mediators of cellular function, upregulation of apoptosis and activation of TGF-β/SMAD3 signaling pathway in pathogenic T-cells favored reduced ability to kill β-cells. These data provide novel evidence that initiating β-cell proliferation, alone, prior to islet infiltration by immune cells alters the identity of β-cells, decreases pathologic self-reactivity of effector cells and increases Tregs to prevent progression of T1D.

2019-03-29 | GSE128315 | GEO

Effects of increased intracellular calcium and overnutrition on pancreatic beta cell transcriptome

Project description:To determine the individual and combined effects of excitotoxicity and overnutrition on beta-cell gene expression, we analyzed the impact of a high fat diet (HFD) on wild type C57Bl/6J and Abcc8 knockout mice of different sexes. We collected 31 bulk RNA-Seq datasets that originated from FACS-purified pancreatic beta-cells that differed by genotype, diet, and sex.

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Gene expression of PD-L1 high and low pancreatic beta cells, after 20h of incubation wiht 50pg.mL of IFNγ.

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MicroRNA profiling of human pancreatic alpha and beta cells

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Expression data from pancreatic beta cells isolated from differentially weaned mice.

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2013-01-30 | E-GEOD-38360 | biostudies-arrayexpress

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