Proteomics

Dataset Information

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Go_BioID_humancellmap_HEK293_lowSDS_2019

ABSTRACT: This dataset consists of 68 raw MS files and associated peak lists and result files, acquired on Velos Orbitrap Elite mass spectrometer operated in Data Dependent Acquisition mode. All samples are BioID experiments, performed in HEK293 Flp-In T-REx cells from organellar and other subcellular markers using a protocol with low concentrations of SDS for lysis and washes. Sample preparation and mass spectrometric acquisition were performed by Christopher Go. The files are associated with a manuscript by Go et al. that describes generation of a proximity-dependent biotinylation (BioID) map of a human cell. Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000084359 | MassIVE |

SECONDARY ACCESSION(S): PXD015530

REPOSITORIES: MassIVE

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Publications

Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy<sup>1</sup> and biochemical fractionation coupled with mass spectrometry<sup>2-4</sup> have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define t  ...[more]

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