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ABSTRACT: Transcriptional response of Drosophila cells to FHV RNA replication

OTHER RELATED OMICS DATASETS IN: E-GEOD-15466

PROVIDER: PRJNA115907 | ENA |

REPOSITORIES: ENA

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Transcription profiling of Drosophila cells response to FHV RNA replication

Project description:This SuperSeries is composed of the following subset Series:; GSE15466: Transcriptional response of Drosophila cells to FHV infection (infection experiment); GSE15467: Transcriptional response of Drosophila cells to FHV RNA1 replicon expression (replicon experiment) Experiment Overall Design: Refer to individual Series

2009-04-10 | E-GEOD-15469 | biostudies-arrayexpress

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Project description:Transcriptional response of Drosophila cells to FHV infection (infection experiment)

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Transcriptional response of Drosophila cells to FHV infection (infection experiment)

Project description:Virus infections induce cellular gene up and down regulation, and these changes often provide clues to cellular pathways utilized by viruses. We used microarrays to examine the transcriptional responses of cultured Drosophila S2 cells to infection with Flock House virus (FHV). Experiment Overall Design: Cultured S2 cells were infected with FHV at an MOI of 10 and we measured global transcript levels at 12 h after infection compared to control mock infected cells using Affymetrix Drosophila Genome 1.0 microarray chips.

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Project description:Virus infections induce cellular gene up and down regulation, and these changes often provide clues to cellular pathways utilized by viruses. We used microarrays to examine the transcriptional responses of cultured Drosophila S2 cells to Flock House virus (FHV) replicon induction. Experiment Overall Design: Cultured S2 cells stably transfected with either a control replicon (pS2F1fs) or an FHV RNA1 replicon (pS2F1) were induced with 1 mM copper and we measured global transcript levels at 18 h after induction using Affymetrix Drosophila Genome 2.0 microarray chips.

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Project description:We focus our analyses on the description of viral small RNAs (FHVdeltaB2 or FHV) and based on genetic and molecular evidende, classify them and discuss their relevance in antiviral defense. Small RNAs from adult Drosophila flies from different genetic backgrounds and infected with FHV-deltaB2 or FHV were sequenced using the illumina platform.

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