Ontology highlight
ABSTRACT:
PROVIDER: PRJNA740107 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures. To maximize PHA production, mixed microbial cultures may be enriched for PHA-producing bacteria with a high storage capacity through the imposition of cyclic, aerobic feast-famine conditions in a sequencing batch reactor (SBR). Though enrichment SBRs have been extensively investigated a bulk solutions-level, little evidence at the proteome level is available to describe the observed SBR behavior to guide future SBR optimization strategies. As such, the purpose of this investigation was to characterize proteome dynamics of a mixed microbial culture in an SBR operated under aerobic feast-famine conditions using fermented dairy manure as the feedstock for PHA production. At the beginning of the SBR cycle, excess PHA precursors were provided to the mixed microbial culture (i.e., feast), after which followed a long duration devoid of exogenous substrate (i.e., famine). Two-dimensional electrophoresis was used to separate protein mixtures during a complete SBR cycle, and proteins of interest were identified.
2017-01-31 | PXD003126 | Pride
Project description:Experimental design: 2 genotypes: PI230970 (resistant USDA Plant Introduction (PI) line containing SBR Rpp2 resistance gene) & Embrapa-48 (susceptible Brazilian cultivar) 2 treatments: Soybean rust challenge & mock infection 3 replications 10 time points: 6, 12, 18, 24, 36, 48, 72, 96, 120, 168hai TOTAL: 120 Affymetrix GeneChip(R) Soybean Genome Arrays ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel. The equivalent experiment is GM2 at PLEXdb.] genotype: PI230970 - time: 006 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 006 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 012 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 012 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 018 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 018 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 024 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 024 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 036 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 036 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 048 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 048 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 072 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 072 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 096 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 096 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 120 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 120 - Treated or untreated: Mock(3-replications); genotype: PI230970 - time: 168 - Treated or untreated: SBR(3-replications); genotype: PI230970 - time: 168 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 006 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 006 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 012 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 012 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 018 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 018 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 024 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 024 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 036 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 036 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 048 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 048 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 072 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 072 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 096 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 096 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 120 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 120 - Treated or untreated: Mock(3-replications); genotype: Embrapa48 - time: 168 - Treated or untreated: SBR(3-replications); genotype: Embrapa48 - time: 168 - Treated or untreated: Mock(3-replications)
2011-11-22 | E-GEOD-33410 | biostudies-arrayexpress
Project description:Comparison of one-stage and two-stage Anammox bioreactor microbial communities Metagenomic assembly
| PRJNA743350 | ENA
Project description:Comparison of one-step and two-step PCR for 16S Illumina MiSeq
| PRJEB43472 | ENA
Project description:Comparison of one-step and two-step PCR for 16S Illumina MiSeq
| PRJEB48796 | ENA
Project description:Next-Generation-Sequencing (NGS) technologies have led to important improvement in the detection of new or unrecognized infective agents, related to infectious diseases. In this context, NGS high-throughput technology can be used to achieve a comprehensive and unbiased sequencing of the nucleic acids present in a clinical sample (i.e. tissues). Metagenomic shotgun sequencing has emerged as powerful high-throughput approaches to analyze and survey microbial composition in the field of infectious diseases. By directly sequencing millions of nucleic acid molecules in a sample and matching the sequences to those available in databases, pathogens of an infectious disease can be inferred. Despite the large amount of metagenomic shotgun data produced, there is a lack of a comprehensive and easy-use pipeline for data analysis that avoid annoying and complicated bioinformatics steps. Here we present HOME-BIO, a modular and exhaustive pipeline for analysis of biological entity estimation, specific designed for shotgun sequenced clinical samples. HOME-BIO analysis provides comprehensive taxonomy classification by querying different source database and carry out main steps in metagenomic investigation. HOME-BIO is a powerful tool in the hand of biologist without computational experience, which are focused on metagenomic analysis. Its easy-to-use intrinsic characteristic allows users to simply import raw sequenced reads file and obtain taxonomy profile of their samples.
2021-07-26 | E-MTAB-9189 | biostudies-arrayexpress
Project description:Next-Generation-Sequencing (NGS) technologies have led to important improvement in the detection of new or unrecognized infective agents, related to infectious diseases. In this context, NGS high-throughput technology can be used to achieve a comprehensive and unbiased sequencing of the nucleic acids present in a clinical sample (i.e. tissues). Metagenomic shotgun sequencing has emerged as powerful high-throughput approaches to analyze and survey microbial composition in the field of infectious diseases. By directly sequencing millions of nucleic acid molecules in a sample and matching the sequences to those available in databases, pathogens of an infectious disease can be inferred. Despite the large amount of metagenomic shotgun data produced, there is a lack of a comprehensive and easy-use pipeline for data analysis that avoid annoying and complicated bioinformatics steps. Here we present HOME-BIO, a modular and exhaustive pipeline for analysis of biological entity estimation, specific designed for shotgun sequenced clinical samples. HOME-BIO analysis provides comprehensive taxonomy classification by querying different source database and carry out main steps in metagenomic investigation. HOME-BIO is a powerful tool in the hand of biologist without computational experience, which are focused on metagenomic analysis. Its easy-to-use intrinsic characteristic allows users to simply import raw sequenced reads file and obtain taxonomy profile of their samples.
2021-07-26 | E-MTAB-9191 | biostudies-arrayexpress