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SILAC study on the effect of trans fatty acids in HepG2 cells

ABSTRACT: The response of HepG2-SF cells exposed to 100 µM fatty acids during 7 days was investigated using stable isotope labeling by amino acids in cell culture (SILAC) for analysis of the full proteome

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver, Hepg2 Cell

SUBMITTER: Lone Vendel Nielsen

LAB HEAD: Jan J. Enghild

PROVIDER: PXD000760 | Pride | 2015-12-14

REPOSITORIES: Pride

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			Action	DRS
	120705_LVN_EC_Gel_18.dat	Other
	120705_LVN_EC_Gel_18.dat-pride.pride.mgf.gz	Mgf
	120705_LVN_EC_Gel_18.dat-pride.pride.mztab.gz	Mztab
	120705_LVN_EC_Gel_18.dat-pride.xml.gz	Xml
	120705_LVN_EC_Gel_18.mgf	Mgf

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Publications

Hepatocytes respond differently to major dietary trans fatty acid isomers, elaidic acid and trans-vaccenic acid.

Krogager Toke P TP Nielsen Lone Vendel LV Kahveci Derya D Dyrlund Thomas F TF Scavenius Carsten C Sanggaard Kristian W KW Enghild Jan J JJ

Proteome science 20151201

<h4>Background</h4>It has been discussed if the adverse health effect associated with the ingestion of trans fatty acids correlates with the food source, as the composition of the isomers varies in different foods. We have investigated the hepatocellular responses to the predominant trans fatty acid isomers in industrially produced partially hydrogenated vegetable oils (elaidic acid) and products of ruminant origin (trans-vaccenic acid).<h4>Results</h4>The responses of HepG2-SF cells exposed to ...[more]

PMID: 26628894

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