Proteomics

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Dissection of IL-2 and IL-15 signaling pathways

ABSTRACT: The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we combined triple SILAClabeling of leukemic T-cells with phosphotyrosine (pY)-specific antibody-based protein enrichment followed by mass spectrometry (MS) analysis. Following the strategy described above, we managed to decipher in detail the complex signaling networks triggered by IL-2 and IL-15. Interestingly, a large number of components of the three main signaling pathways known to be initiated in response to the interleukins (JAK/STAT; RAS/MAPK; PI3K/AKT) were identified.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell

DISEASE(S): Acute Leukemia

SUBMITTER: Nerea Osinalde  

LAB HEAD: Nerea Osinalde

PROVIDER: PXD001129 | Pride | 2014-09-23

REPOSITORIES: Pride

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20130122NO_va2g140_0121a25r_pIP12_TiO2_1.raw Raw
20130122NO_va2g140_0121a25r_pIP12_TiO2_2.raw Raw
20130122NO_va2g140_0121a25r_pIP12_TiO2_3A.raw Raw
20130122NO_va2g140_0121a25r_pIP12_TiO2_3B.raw Raw
20130122NO_va2g140_0121a25r_pIP12_TiO2_4A.raw Raw
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Publications

Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics.

Osinalde Nerea N   Sanchez-Quiles Virginia V   Akimov Vyacheslav V   Guerra Barbara B   Blagoev Blagoy B   Kratchmarova Irina I  

Proteomics 20140930 2-3

Common γ-chain family of cytokines (IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, where IL stands for interleukin) are key regulators of the immune homeostasis that exhibit pleiotropic biological activities and even sometimes redundant roles as a result of the utilization of the same receptor subunit. However, they also exert distinct functions that make each of them to be indispensable. For instance, all family members can act as T-cell growth factors; however, we found that IL-15 but not IL-7 can  ...[more]

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