Proteomics

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The Quantitative Changes in the Yeast Hsp70 and Hsp90 Interactomes upon DNA Damage

ABSTRACT: The molecular chaperones Hsp70 and Hsp90 participate in many important cellular processes, including how cells respond to DNA damage. In this study, we applied quantitative affinity-purification mass spectrometry (AP-MS) proteomics to understand the protein network through which Hsp70 and Hsp90 exert their effects on the DNA damage response (DDR). We characterized the interactomes of the yeast Hsp70 isoform Ssa1 and Hsp90 isoform Hsp82 before and after exposure to methyl methanesulfonate. We identified 256 chaperone interactors, 146 of which are novel. Although the majority of chaperone interaction remained constant under DNA damage, 5 proteins (Coq5, Ast1, Cys3, Ydr210c and Rnr4) increased in interaction with Ssa1 and/or Hsp82. This data is related to article “Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveals chaperone-dependent regulation of ribonucleotide reductase”.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Donald Wolfgeher  

LAB HEAD: Stephen J. Kron

PROVIDER: PXD001284 | Pride | 2014-12-08

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
HSP82_A_18O_Fwd_Rev.zip Other
HSP82_B_18O_Fwd_Rev.zip Other
HSP82_C_18O_Fwd_Rev.zip Other
SSA1_A_18O_Fwd_Rev.zip Other
SSA1_B_18O_Fwd_Rev.zip Other
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Publications

Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase.

Truman Andrew W AW   Kristjansdottir Kolbrun K   Wolfgeher Donald D   Ricco Natalia N   Mayampurath Anoop A   Volchenboum Samuel L SL   Clotet Josep J   Kron Stephen J SJ  

Journal of proteomics 20141018

The highly conserved molecular chaperones Hsp90 and Hsp70 are indispensible for folding and maturation of a significant fraction of the proteome, including many proteins involved in signal transduction and stress response. To examine the dynamics of chaperone-client interactions after DNA damage, we applied quantitative affinity-purification mass spectrometry (AP-MS) proteomics to characterize interactomes of the yeast Hsp70 isoform Ssa1 and Hsp90 isoform Hsp82 before and after exposure to methy  ...[more]

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