Proteomics

Dataset Information

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Middle-down MS analysis of intact and clipped histone H3 proteoforms


ABSTRACT: There is growing evidence that proteolytic cleavage of histone N-terminal tails, known as histone clipping, influences nucleosome dynamics and functional properties. Using middle-down MS analysis we characterized intact and proteolytically processed histone H3 proteoforms isolated from human hepatocarcinoma cell line HepG2/C3A grown in 3D culture.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Andrey Tvardovskiy  

LAB HEAD: Ole Nørregaard Jensen

PROVIDER: PXD002535 | Pride | 2022-03-14

REPOSITORIES: Pride

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Publications

Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns.

Tvardovskiy Andrey A   Wrzesinski Krzysztof K   Sidoli Simone S   Fey Stephen J SJ   Rogowska-Wrzesinska Adelina A   Jensen Ole N ON  

Molecular & cellular proteomics : MCP 20150930 12


Post-translational modifications (PTMs) of histone proteins play a fundamental role in regulation of DNA-templated processes. There is also growing evidence that proteolytic cleavage of histone N-terminal tails, known as histone clipping, influences nucleosome dynamics and functional properties. Using top-down and middle-down protein analysis by mass spectrometry, we report histone H2B and H3 N-terminal tail clipping in human hepatocytes and demonstrate a relationship between clipping and co-exi  ...[more]

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