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Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury: control, guanidine

ABSTRACT: The aim of the present study is to characterize ECM remodeling after myocardial infarction by applying a proteomics method based on ECM enrichment and MS analysis to a porcine model of ischemia/reperfusion injury. The human-like physiology and anatomy of pigs facilitate comparisons of different cardiac regions to establish an accurate temporal and spatial pattern of the ECM remodeling process.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Sus Scrofa (pig)

TISSUE(S): Heart

DISEASE(S): Myocardial Ischemia

SUBMITTER: Javier Barallobre-Barreiro

LAB HEAD: Manuel Mayr

PROVIDER: PXD003918 | Pride | 2016-04-07

REPOSITORIES: Pride

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			Action	DRS
	RGCA_01.RAW	Raw
	RGCA_02.RAW	Raw
	RGCA_03.RAW	Raw
	RGCA_04.RAW	Raw
	RGCA_05.RAW	Raw

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Publications

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.

Barallobre-Barreiro Javier J Didangelos Athanasios A Schoendube Friedrich A FA Drozdov Ignat I Yin Xiaoke X Fernández-Caggiano Mariana M Willeit Peter P Puntmann Valentina O VO Aldama-López Guillermo G Shah Ajay M AM Doménech Nieves N Mayr Manuel M

Circulation 20120118 6

<h4>Background</h4>After myocardial ischemia, extracellular matrix (ECM) deposition occurs at the site of the focal injury and at the border region.<h4>Methods and results</h4>We have applied a novel proteomic method for the analysis of ECM in cardiovascular tissues to a porcine model of ischemia/reperfusion injury. ECM proteins were sequentially extracted and identified by liquid chromatography tandem mass spectrometry. For the first time, ECM proteins such as cartilage intermediate layer prote ...[more]

PMID: 22261194

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2016-04-07 | PXD003919 | Pride

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