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Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury Guanidine Infarct, focal area

ABSTRACT: The aim of the present study is to characterize ECM remodeling after myocardial infarction by applying a proteomics method based on ECM enrichment and MS analysis to a porcine model of ischemia/reperfusion injury. The human-like physiology and anatomy of pigs facilitate comparisons of different cardiac regions to establish an accurate temporal and spatial pattern of the ECM remodeling process.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Sus Scrofa (pig)

TISSUE(S): Heart

DISEASE(S): Myocardial Ischemia

SUBMITTER: Javier Barallobre-Barreiro

LAB HEAD: MAnuel Mayr

PROVIDER: PXD003926 | Pride | 2016-04-11

REPOSITORIES: Pride

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			Action	DRS
	IGIA_01.RAW	Raw
	IGIA_02.RAW	Raw
	IGIA_03.RAW	Raw
	IGIA_04.RAW	Raw
	IGIA_05.RAW	Raw

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Publications

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.

Barallobre-Barreiro Javier J Didangelos Athanasios A Schoendube Friedrich A FA Drozdov Ignat I Yin Xiaoke X Fernández-Caggiano Mariana M Willeit Peter P Puntmann Valentina O VO Aldama-López Guillermo G Shah Ajay M AM Doménech Nieves N Mayr Manuel M

Circulation 20120118 6

<h4>Background</h4>After myocardial ischemia, extracellular matrix (ECM) deposition occurs at the site of the focal injury and at the border region.<h4>Methods and results</h4>We have applied a novel proteomic method for the analysis of ECM in cardiovascular tissues to a porcine model of ischemia/reperfusion injury. ECM proteins were sequentially extracted and identified by liquid chromatography tandem mass spectrometry. For the first time, ECM proteins such as cartilage intermediate layer prote ...[more]

PMID: 22261194

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Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury: control, guanidine

Project description:The aim of the present study is to characterize ECM remodeling after myocardial infarction by applying a proteomics method based on ECM enrichment and MS analysis to a porcine model of ischemia/reperfusion injury. The human-like physiology and anatomy of pigs facilitate comparisons of different cardiac regions to establish an accurate temporal and spatial pattern of the ECM remodeling process.

2016-04-07 | PXD003918 | Pride

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury: Infarcted, Guanidine

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In depth quantification of extracellular matrix proteins from human pancreas

Project description:Extracellular matrix (ECM) is an important component of the pancreatic microenvironment which regulates β cell proliferation, differentiation and insulin secretion. Protocols have recently been developed for the decellularization of the human pancreas to generate functional scaffolds and hydrogels. In this work, we characterized human pancreatic ECM composition before and after decellularization using isobaric dimethylated leucine (DiLeu) labeling for relative quantification of ECM proteins. A novel correction factor was employed in the study to eliminate the bias introduced during sample preparation. In comparison to the commonly employed proteomic approaches (urea and FASP), a recently developed surfactant and chaotropic agent assisted sequential extraction/on pellet digestion (SCAD) protocol was proven to be a superior strategy for ECM protein extraction of human pancreatic ECM matrix. The quantitative proteomic results revealed the preservation of matrisome proteins while most of the cellular proteins were removed. This method was compared with a well-established label-free quantification (LFQ) approach which rendered similar expressions of different categories of proteins (collagens, ECM glycoproteins, proteoglycans, etc.). The distinct expression of ECM proteins was quantified comparing adult and fetal pancreas ECM, shedding light on the correlation between matrix composition and post-natal β cell maturation. Despite the distinct profiles of different subcategories in the native pancreas, the distribution of matrisome protein exhibited similar trends after the decellularization process. Our method generates the largest dataset of matrisome proteins from a single tissue type. These results provide valuable insight into the possibilities of constructing a bioengineered pancreas. It also facilitates an understanding of the significant roles that matrisome proteins play in post-natal cell maturation.

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Impact of fibrillar collagen scaffold on corneal stromal fibroblasts protein expression

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Distinct Combinatorial Cascade of Events Generated by ECM Proteolysis Dictates Cell Behavior

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2016-09-27 | PXD003553 | Pride

Extracellular Matrix Proteomics Identifies Molecular Signature of Atherosclerotic Plaques from Symptomatic Patients

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ECM deposition is driven by caveolin1-dependent regulation of exosomal biogenesis and cargo sorting

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