Proteomics

Dataset Information

0

Architecture of the yeast Elongator complex

ABSTRACT: The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1-6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub-complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2 and Elp3 subunits form a two-lobed scaffold, which binds Elp456 asymmetrically. Our topological models are consistent with previous studies on individual subunits and further validated by complementary biochemical analyses. Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Alessandro Ori  

LAB HEAD: Martin Beck

PROVIDER: PXD005251 | Pride | 2016-12-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
AO-A286.raw Raw
AO-A287.raw Raw
AO-A288.raw Raw
AO-A289.raw Raw
AO-A290.raw Raw
Items per page:
1 - 5 of 66
altmetric image

Publications

The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1-6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub-complex solved  ...[more]

Similar Datasets

Proteomics Integrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints
2019-12-10 | PXD016639 | Pride
Proteomics RNxQuest: an extension to the xQuest pipeline enabling analysis of protein-RNA cross-linking/mass spectrometry data
2023-09-06 | PXD039754 | Pride
Proteomics,Multiomics Crosslinking mass spectrometry of yeast Pol I pre-initiation complex
2017-07-31 | PXD006510 | Pride
Genomics Tbr2 and Neurog2 transcription factor binding sites in E14.5 WT cerebral cortices
2016-09-08 | E-GEOD-63620 | biostudies-arrayexpress
Proteomics Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains
2016-01-04 | PXD002234 | Pride
Proteomics Cross-linking MS data of CtNup82-Y complex
2018-10-17 | PXD007043 | Pride
Transcriptomics Tbr2 and Neurog2 occupancy and transcriptional profiling of control and Tbr2 knockout E14.5 cerebral cortices
2016-09-08 | E-GEOD-63621 | biostudies-arrayexpress
Proteomics Crosslinking-MS dataset of the TFIIIC subcomplex tauA with Brf1 and TBP
2020-12-04 | PXD018232 | Pride
Genomics Deep sequencing of HetR-bound DNA reveals novel HetR targets in Anabaena sp. strain PCC 7120
2013-10-31 | E-GEOD-51886 | biostudies-arrayexpress
Proteomics Distinct roles of the two Elongator subcomplexes during neurodevelopment
2022-05-17 | PXD032925 | Pride