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Identification of protein interaction partners of the lncRNA CALA

ABSTRACT: The stimuli-responsive lncRNA CALA impacts endothelial cell function as silencing impairs angiogenic capacity in vitro. To uncover the molecular mechanism of CALA, we here combined antisense affinity selection of CALA-RNPs with mass spectrometry to identify the protein interactome of CALA. We therefore specifically enrich CALA-RNPs using two distinct antisense probes targeting CALA (LFN – probe#1, LF – probe #2) over a scrambled, non-target control (Scr/NTC) and thereby uncover CALA to interact with components of ribonucleoprotein complexes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig

LAB HEAD: Nicolas Jaé

PROVIDER: PXD033516 | Pride | 2022-07-04

REPOSITORIES: Pride

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			Action	DRS
	Data_analysis.xlsx	Xlsx
	P19_051_Luisa_LK10_Scr_MS5.raw	Raw
	P19_051_Luisa_LK11_New_MS5.raw	Raw
	P19_051_Luisa_LK12_LF_MS5.raw	Raw
	P19_051_Luisa_LK13_Scr_MS6.raw	Raw

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