Proteomics

Dataset Information

0

Polylysine interactome in human cells


ABSTRACT: Polylysine chains are synthesized by ribosomes when a stop codon preceding the polyA tail on the mRNA fails to terminate the translation. To clarify the faith of the polylysine-tagged chains, we analyzed polylysine interactome in the cytosol of human 293T cells. We found an extensive network of interactors involving key complexes of protein quality control, such as TRiC and 26S proteasome. The presence of components of the nuclear transport machinery among polylysine interactors and an enhanced nuclear import suggest a mechanism for compartmentalization of faulty proteins in the cell.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Kidney

SUBMITTER: Giulia Calloni  

LAB HEAD: R. Martin Vabulas

PROVIDER: PXD008171 | Pride | 2018-04-26

REPOSITORIES: Pride

altmetric image

Publications

Polylysine is a Proteostasis Network-Engaging Structural Determinant.

Lang Wei-Han WH   Lang Wei-Han WH   Calloni Giulia G   Vabulas R Martin RM  

Journal of proteome research 20180425 5


C-terminal polylysine (PL) can be synthesized from the polyadenine tail of prematurely cleaved mRNAs or when a read-though of a stop codon happens. Due to the highly positive charge, PL stalls in the electrostatically negative ribosomal exit channel. The stalled polypeptide recruits the Ribosome-associated quality control (RQC) complex which processes and extracts the nascent chain. Dysfunction of the RQC leads to the accumulation of PL-tagged proteins, induction of a stress response, and cellul  ...[more]

Similar Datasets

2022-10-15 | PXD033469 | Pride
2013-12-04 | E-GEOD-52808 | biostudies-arrayexpress
2024-11-04 | PXD045888 | Pride
2018-01-29 | PXD006575 | Pride
2024-01-31 | PXD043878 | Pride
2019-06-28 | PXD012945 | Pride
2016-07-27 | E-GEOD-71990 | biostudies-arrayexpress
2013-05-10 | E-GEOD-41235 | biostudies-arrayexpress
2014-02-19 | E-GEOD-55122 | biostudies-arrayexpress
2015-03-30 | E-GEOD-60498 | biostudies-arrayexpress