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SPD1590_iTRAQ - A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties

ABSTRACT: Streptococcus pneumoniae has three main transporters for iron acquisition: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in a piuA/piaA/pitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present work, using spd1590-deleted and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Streptococcus Pneumoniae Serotype 2 (strain D39 / Nctc 7466)

TISSUE(S): Cell Culture

SUBMITTER: Xin Yu Miao

LAB HEAD: Xuesong Sun

PROVIDER: PXD008814 | Pride | 2018-08-08

REPOSITORIES: Pride

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			Action	DRS
	SPD1590_iTRAQ_1.raw	Raw
	SPD1590_iTRAQ_10.raw	Raw
	SPD1590_iTRAQ_11.raw	Raw
	SPD1590_iTRAQ_12.raw	Raw
	SPD1590_iTRAQ_13.raw	Raw

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A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties.

Miao Xinyu X He Jiaojiao J Zhang Liang L Zhao Xinlu X Ge Ruiguang R He Qing-Yu QY Sun Xuesong X

Frontiers in microbiology 20180720

Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present study, using spd1590-knockout, -complemented, and -overexpressin ...[more]

PMID: 30079056

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Project description:Streptococcus pneumoniae (S.pneumoniae) is a Gram-positive bacterial pathogen that colonizes on the mucosal surfaces of the host's nasopharynx and upper respiratory tract and results in pneumonia. In order to survive and infect the host, S.pneumoniae must have obtain essential nutrients, such as transition metal ions . Our previous study had shown that the mRNA and protein levels of SPD-0090 are significantly upregulated in the ΔpiuA/ΔpiaA/ΔpitA triple mutant (three major iron transports), but its detailed biological function is unknown. In this study, we found that the knockout spd-0090 gene induced a delayed growth in the medium with different sugar sources. F urther iTRAQ quantitative proteomics studies revealed that SPD-0090 affects galactose metabolism and iron ion transport system. Then RT-qPCR and intracellular galactose content assays showed that SPD-0090 affects galactose uptake, leads to reduced galactose utilization. In addition, in vitro biochemical assays showed that SPD-0090 is a hemin transporter and affects iron uptake. Notably the knockout of spd-0090 resulted in an enhanced infection ability of S.pneumonaie to Our study reveals that the dual function of SPD-0090 plays an important role in the virulence of S.pneumoniae.

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A Novel Iron Transporter SPD_1590 in Streptococcus pneumoniae Contributing to Bacterial Virulence Properties.

Project description:Streptococcus pneumoniae, a Gram-positive human pathogen, has evolved three main transporters for iron acquisition from the host: PiaABC, PiuABC, and PitABC. Our previous study had shown that the mRNA and protein levels of SPD_1590 are significantly upregulated in the ?piuA/?piaA/?pitA triple mutant, suggesting that SPD_1590 might be a novel iron transporter in S. pneumoniae. In the present study, using spd1590-knockout, -complemented, and -overexpressing strains and the purified SPD_1590 protein, we show that SPD_1590 can bind hemin, probably supplementing the function of PiuABC, to provide the iron necessary for the bacterium. Furthermore, the results of iTRAQ quantitative proteomics and cell-infection studies demonstrate that, similarly to other metal-ion uptake proteins, SPD_1590 is important for bacterial virulence properties. Overall, these results provide a better understanding of the biology of this clinically important bacterium.

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