Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hair Proteome Variation at Different Body Locations on GVP Detection for Protein-Based Human Identification

ABSTRACT: Characterization of body location-specific hair proteomic variation and its effect on the identification of genetically variant peptide markers for protein-based human identification. LLNL-MI-756827

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte

SUBMITTER: Fanny Chu

LAB HEAD: Bradley R. Hart

PROVIDER: PXD010982 | Pride | 2019-05-22

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	QE5171.raw	Raw
	QE5173.raw	Raw
	QE5181.raw	Raw
	QE5329.raw	Raw
	QE5736.raw	Raw

Items per page:

1 - 5 of 108

Publications

Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification.

Chu Fanny F Mason Katelyn E KE Anex Deon S DS Jones A Daniel AD Hart Bradley R BR

Scientific reports 20190521 1

Human hair contains minimal intact nuclear DNA for human identification in forensic and archaeological applications. In contrast, proteins offer a pathway to exploit hair evidence for human identification owing to their persistence, abundance, and derivation from DNA. Individualizing single nucleotide polymorphisms (SNPs) are often conserved as single amino acid polymorphisms in genetically variant peptides (GVPs). Detection of GVP markers in the hair proteome via high-resolution tandem mass spe ...[more]

PMID: 31113963

Similar Datasets

Proteomic characterization of damaged single hairs recovered after an explosion for protein-based human identification

Project description:Characterization of hair proteome in damaged single hairs after an explosive blast and evaluation of success rates in genetically variant peptide marker detection for protein-based human identification.

2020-05-14 | PXD017427 | Pride

Profiling Protein Expression Levels and Genetically Variant Peptides in Human Hair From Different Body Sites

Project description:Proteins of the human hair shaft contain a wealth of information about the coding regions of the person’s genome from whom the hair is derived. Commonly found at crime scenes, hair shafts may thus provide useful forensic evidence if the information they contain can be exploited. Present experiments show that hair shafts from four different anatomic sites are similarly useful in distinguishing individuals by protein profiling. However, the results demonstrated that protein profiles were dependent on anatomic site, indicating that a proper comparison requires matching the sites of origin. The differences in profile offer the prospect of determining the site of origin of hair by comparison with profiles of shafts from other anatomic sites. By contrast, the genetically variant peptides detected in the protein digests, that map to non-synonymous single nucleotide polymorphisms in subject DNA, were detectable regardless of the anatomic origin of the hair shafts. The resulting profiles of genetically variant peptides were more dependent on a subject’s genotype than on the anatomic origin of the hair shaft. Individual identification therefore can be based on peptide profiles regardless of body location. This study demonstrates the utility of proteomic analysis for increasing the forensic value of hair shaft evidence.

2019-04-04 | PXD011732 | Pride

Transcripotome analysis of different locations of hair follicles (bulb, bulge) in androgenetic alopecia

Project description:To fully interrogate location-specific variations of the hair follicle in AGA and normal persons, we sought to perform a thorough and comprehensive transcripotome profiling of different locations of hair follicles (bulb portions, bulge portions) in balding and non-balding areas of AGA, with normal comparisons.

2017-12-25 | GSE101451 | GEO

Hair Proteome Variation at Different Body Locations on Genetically Variant Peptide Detection for Protein-Based Human Identification.

Project description:Human hair contains minimal intact nuclear DNA for human identification in forensic and archaeological applications. In contrast, proteins offer a pathway to exploit hair evidence for human identification owing to their persistence, abundance, and derivation from DNA. Individualizing single nucleotide polymorphisms (SNPs) are often conserved as single amino acid polymorphisms in genetically variant peptides (GVPs). Detection of GVP markers in the hair proteome via high-resolution tandem mass spectrometry permits inference of SNPs with known statistical probabilities. To adopt this approach for forensic investigations, hair proteomic variation and its effects on GVP identification must first be characterized. This research aimed to assess variation in single-inch head, arm, and pubic hair, and discover body location-invariant GVP markers to distinguish individuals. Comparison of protein profiles revealed greater body location-specific variation in keratin-associated proteins and intracellular proteins, allowing body location differentiation. However, robust GVP markers derive primarily from keratins that do not exhibit body location-specific differential expression, supporting GVP identification independence from hair proteomic variation at the various body locations. Further, pairwise comparisons of GVP profiles with 8 SNPs demonstrated greatest interindividual variation and high intraindividual consistency, enabling similar differentiative potential of individuals using single hairs irrespective of body location origin.

| S-EPMC6529471 | biostudies-literature

Next-generation sequencing of microRNAs in different locations of hair follicles (bulb, bugle)in androgenetic alopecia

Project description:To fully interrogate location-specific variations of microRNAs of the hair follicle in AGA and normal persons, we sought to perform a thorough and comprehensive microRNA profiling of different locations of hair follicles (bulb portions, bugle portions)

2018-12-24 | GSE111788 | GEO

Human hair shaft LC-MS/MS - Studies on the Proteome of Human Hair - Identification of Histones and Deamidated Keratins

Project description:The aim of this work was to define the hair proteome after extractions with both detergent and detergent-free buffers using highly sensitive proteomics technologies based on mass spectrometry.

2018-01-30 | PXD007224 | Pride

Transcription profiling of mouse back skin during the hair growth cycle to identify hair cycle-associated genes

Project description:Identification of hair cycle-associated genes from time-course gene expression profile data by using replicate variance. Proc. Natl. Acad. Sci. USA. (2004)

2007-11-02 | E-GEOD-1912 | biostudies-arrayexpress

Less invasive diagnostics for cancer using hair follicle

Project description:Primary outcome(s): Comparison of the gene expression profile in hair follicle between cancer patients and healthy volunteer

| 2628246 | ecrin-mdr-crc

Targets of FGFR2-IIIb signalling in the hair follicle

Project description:rationale: comparison of gene expression profiles in wildtype and Foxn1::dnFGFR2-IIIb transgenic hair follicles; identification of targets that mediate the effects seen in transgenic hair follicles results: as already suggested by the phenotype, the molecular abnormalities appear to be restricted to the hair shaft medulla; Igfbp5 is an important mediator of transgene-dependent effects Keywords: ordered

2005-07-20 | GSE2463 | GEO

Transcription profiling of mouse wildtype and Foxn1::dnFGFR2-IIIb transgenic hair follicles

Project description:rationale: comparison of gene expression profiles in wildtype and Foxn1::dnFGFR2-IIIb transgenic hair follicles; identification of targets that mediate the effects seen in transgenic hair follicles; results: as already suggested by the phenotype, the molecular abnormalities appear to be restricted to the hair shaft medulla; Igfbp5 is an important mediator of transgene-dependent effects

2008-06-12 | E-GEOD-2463 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data