Proteomics

Dataset Information

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Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in Equine osteoarthritis: An exploratory ‘multi-omic’ study


ABSTRACT: Osteoarthritis (OA) causes persistent joint deterioration, severe morbidity, and reduced mobility in both humans and horses. Extracellular vesicles (EVs) are key players in cell-to-cell communication and are thus ideal for biomarker discovery. Here, we used a unique multi-omics strategy to find possible EV biomarkers from the synovial fluid of healthy horses compared to naturally occurring OA and advanced OA. We discovered that SF-EVs’ proteome and phospholipidome are correlated. Furthermore, we found that the quantity of EVs present in SF was unaffected by OA. We discovered crucial signalling pathways involving integrins (p=2.72x10-24), the actin cytoskeleton (p=3.88x10-32), Rho family GTPases (p=1.24x10-19), and the clathrin-mediated endocytosis pathway (p=1.30x10-24). Specifically, the advanced OA group’s immunological response (macrophage binding and interaction) was upregulated. These findings are suggestive that the membrane and protein cargo of SF-EVs are altered in response to OA pathology and may be used as biomarkers of disease. Consequently, SF-EVs could have the potential to be employed in the future to help with OA stratification and prognosis. In addition, this study provides a framework for future research using a larger cohort.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Synovial Fluid

DISEASE(S): Osteoarthritis

SUBMITTER: Rosalind Jenkins  

LAB HEAD: Rosalind Elspeth Jenkins

PROVIDER: PXD042765 | Pride | 2024-05-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
EJC_LVP_SF_EV10_SWATH_131222.wiff Wiff
EJC_LVP_SF_EV10_SWATH_131222.wiff.scan Wiff
EJC_LVP_SF_EV11_SWATH_131222.wiff Wiff
EJC_LVP_SF_EV11_SWATH_131222.wiff.scan Wiff
EJC_LVP_SF_EV12_SWATH_131222.wiff Wiff
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Publications

Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in equine osteoarthritis: An exploratory 'multi-omics' study to identify composite biomarkers.

Clarke Emily E   Varela Laura L   Jenkins Rosalind E RE   Lozano-Andrés Estefanía E   Cywińska Anna A   Przewozny Maciej M   van Weeren P René PR   van de Lest Chris H A CHA   Peffers Mandy M   Wauben Marca H M MHM  

Biochemistry and biophysics reports 20240118


Osteoarthritis causes progressive joint deterioration, severe morbidity, and reduced mobility in both humans and horses. Currently, osteoarthritis is diagnosed at late stages through clinical examination and radiographic imaging, hence it is challenging to address and provide timely therapeutic interventions to slow disease progression or ameliorate symptoms. Extracellular vesicles are cell-derived vesicles that play a key role in cell-to-cell communication and are potential sources for specific  ...[more]

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