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Middle-Down Proteomic Analyses with Ion Mobility Separations of Endogenous Isomeric Proteoforms

ABSTRACT: We integrated FAIMS into the WCX-HILIC ETD MS/MS system to improve identification of histonetail proteforms

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Early Embryonic Cell

SUBMITTER: Pavel Shliaha

LAB HEAD: Ole N Jensen

PROVIDER: PXD016918 | Pride | 2020-04-02

REPOSITORIES: Pride

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			Action	DRS
	FAIMS_1.csv	Csv
	FAIMS_2.csv	Csv
	FAIMS_3.csv	Csv
	FAIMS_4.csv	Csv
	FAIMS_5.csv	Csv

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Middle-Down Proteomic Analyses with Ion Mobility Separations of Endogenous Isomeric Proteoforms.

Shliaha Pavel V PV Gorshkov Vladimir V Kovalchuk Sergey I SI Schwämmle Veit V Baird Matthew A MA Shvartsburg Alexandre A AA Jensen Ole N ON

Analytical chemistry 20200117 3

Biological functions of many proteins are governed by post-translational modifications (PTMs). In particular, the rich PTM complement in histones controls the gene expression and chromatin structure with major health implications via a combinatoric language. Deciphering that "histone code" is the great challenge for proteomics given an astounding number of possible proteoforms, including isomers with different PTM positions. These must be disentangled on the top- or middle-down level to preserve ...[more]

PMID: 31935065

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