Dataset Information

Proteomic characterization of probiotics

ABSTRACT: The manufacturing processes of commercial probiotic strains may be affected in different ways in the attempt to optimize yield, costs, functionality, or stability, influencing gene expression, protein patterns, or metabolic output. Aim of this work was to compare different samples of a high concentration (450 billion bacteria) multispecies (8 strains) formulation produced at two different manufacturing sites, USA (US) and Italy (IT), by applying functional proteomics analyses. Several protein-profile differences were detected between IT- and US-made products, with Lactobacillus paracasei, Streptococcus thermophilus, and Bifidobacteria being the main affected probiotics/microorganisms. Integration of omics platforms with in vivo analysis emerged as a powerful tool to assess manufacturing procedures.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Lactobacillus Acidophilus Ncfm Bacteria

SUBMITTER: Benedetta Mattei

LAB HEAD: Benedetta Mattei

PROVIDER: PXD017086 | Pride | 2020-01-22

REPOSITORIES: Pride

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Publications

A Combined Proteomics, Metabolomics and In Vivo Analysis Approach for the Characterization of Probiotics in Large-Scale Production.

Bianchi Laura L Laghi Luca L Correani Virginia V Schifano Emily E Landi Claudia C Uccelletti Daniela D Mattei Benedetta B

Biomolecules 20200118 1

The manufacturing processes of commercial probiotic strains may be affected in different ways in the attempt to optimize yield, costs, functionality, or stability, influencing gene expression, protein patterns, or metabolic output. Aim of this work is to compare different samples of a high concentration (450 billion bacteria) multispecies (8 strains) formulation produced at two different manufacturing sites, United States of America (US) and Italy (IT), by applying a combination of functional pr ...[more]

PMID: 31963736

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