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Changes in bovine Lymphocyte proteome after stimulation with pokeweed mitogen

ABSTRACT: A new vaccine against bovine viral diarrhea (BVD) induced unwanted immune reactions with production of alloreactive antibodies in 5–10% of vaccinated cows. Transfer of these antibodies via colostrum caused bovine neonatal pancytopenia (BNP) in calves, with a lethality rate of 90%. The exact immunological mechanisms behind the onset of BNP are not fully understood to date, however, an immune deviant phenotype of immune cells is assumed. To gain deeper insight into disease onset and pathogenesis, we analyzed the lymphocyte (PBL) proteome from alloreactive antibody producers (BNP cows) and non-responders (controls) after stimulation with PWM.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Leukocyte, Blood

SUBMITTER: Roxane Degroote

LAB HEAD: Cornelia Annette Deeg

PROVIDER: PXD017350 | Pride | 2022-02-10

REPOSITORIES: Pride

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			Action	DRS
	FRK6597A11MA2-40-239322.raw	Raw
	FRK6597A12MA2-40-239326.raw	Raw
	FRK6597A2MA2-40-239280.raw	Raw
	FRK6597A3MA2-40-239283.raw	Raw
	FRK6597A5MA2-40-239293.raw	Raw

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altered bovine lymphocyte secretome after pokeweed mitogen stimulation

Project description:Vaccination with a new vaccine against bovine viral diarrhea (BVD) caused unwanted immune reactions through alloreactive antibody production in 5–10% of vaccinated cows. Transfer of these alloreactive antibodies via colostrum caused highly lethal (90%) bovine neonatal pancytopenia (BNP) in calves. Despite the assumption of an immune deviant lymphocyte (PBL) phenotype, the exact immunological mechanisms driving these unwanted immune reactions are not fully understood to date. To gain deeper insight into these mechanisms, we analyzed the PBL secretome from alloreactive antibody producers (BNP cows) and non-alloreactive responders (controls) after stimulation with pokeweed mitogen (PWM).

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