Dataset Information

Proteomic and phosphoproteomic changes upon overexpression of S. cerevisiae Ppz1 protein phosphatase

ABSTRACT: Over-expression of Ppz1 is deleterious for yeast cells. Recent studies in our laboratory demonstrated that the over-expression of a catalytically impaired version of Ppz1 (Ppz1R451L) is not toxic, suggesting that the excess of Ppz1 phosphatase activity was responsible for toxicity, possibly due to changes in yeast phosphoproteome. Because the molecular basis of this toxicity are still unknown, a global yeast phosphoproteomic assay was designed to gain insight into this phenomenon. To identify revelant changes cuased by Ppz1 overexpression we have constructed a strain (ZCZ01) in which the PPZ1 promoter has been replaced by the GAL promotrer. Cell grown on raffinose were shifted to galactose and samples were taken after 30, 60, 120 and 240 min and changes in proteome and phosphoproteome were analyzed by LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Diego Velázquez

LAB HEAD: Joaquin Ariño Carmona

PROVIDER: PXD018100 | Pride | 2020-10-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	QEHF3_03138_KS_BSA_EC75-70In2-9-ac_DiegoS1_1.raw	Raw
	QEHF3_03143_KS_BSA_EC75-70In2-1_DiegoP1_1.raw	Raw
	QEHF3_05731_KS_YeastDiegoS2TMT_5fr_fr1.raw	Raw
	QEHF3_05732_KS_YeastDiegoS2TMT_5fr_fr2.raw	Raw
	QEHF3_05733_KS_YeastDiegoS2TMT_5fr_fr3.raw	Raw

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Publications

Yeast Ppz1 protein phosphatase toxicity involves the alteration of multiple cellular targets.

Velázquez Diego D Albacar Marcel M Zhang Chunyi C Calafí Carlos C López-Malo María M Torres-Torronteras Javier J Martí Ramón R Kovalchuk Sergey I SI Pinson Benoit B Jensen Ole N ON Daignan-Fornier Bertrand B Casamayor Antonio A Ariño Joaquín J

Scientific reports 20200924 1

Control of the protein phosphorylation status is a major mechanism for regulation of cellular processes, and its alteration often lead to functional disorders. Ppz1, a protein phosphatase only found in fungi, is the most toxic protein when overexpressed in Saccharomyces cerevisiae. To investigate the molecular basis of this phenomenon, we carried out combined genome-wide transcriptomic and phosphoproteomic analyses. We have found that Ppz1 overexpression causes major changes in gene expression, ...[more]

PMID: 32973189

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