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Validation of the expression of clathrin heavy chain muscle isoform including exon 31 at the protein level

ABSTRACT: A single exon alternative splicing in the CLTC gene determines clathrin coat organization. This event controls plasticity from clathrin-coated pits to plaques, structures that are essential for muscle fiber function and maintenance

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Myoblast, Muscle, Myotube

SUBMITTER: Valentin SABATET

LAB HEAD: Damarys Loew

PROVIDER: PXD018333 | Pride | 2020-06-19

REPOSITORIES: Pride

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			Action	DRS
	Design.xls	Xls
	H3977FD.msf	Msf
	H3977FD.raw	Raw
	H3978FD.msf	Msf
	H3978FD.raw	Raw

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1 - 5 of 21

Publications

Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.

Moulay Gilles G Lainé Jeanne J Lemaître Mégane M Nakamori Masayuki M Nishino Ichizo I Caillol Ghislaine G Mamchaoui Kamel K Julien Laura L Dingli Florent F Loew Damarys D Bitoun Marc M Leterrier Christophe C Furling Denis D Vassilopoulos Stéphane S

The Journal of cell biology 20200901 9

Clathrin function directly derives from its coat structure, and while endocytosis is mediated by clathrin-coated pits, large plaques contribute to cell adhesion. Here, we show that the alternative splicing of a single exon of the clathrin heavy chain gene (CLTC exon 31) helps determine the clathrin coat organization. Direct genetic control was demonstrated by forced CLTC exon 31 skipping in muscle cells that reverses the plasma membrane content from clathrin plaques to pits and by promoting exon ...[more]

PMID: 32642759

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Quaking is a global regulator of muscle-specific alternative splicing in vertebrates

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