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The Caveolin3 complex stabilizes functional surface expression of the Lactate-Proton Shuttle McT1 but not the sodium channel Nav1.5 in cardiomyocytes - Part 2: Ratiometric Proximity Proteomics

ABSTRACT: Caveolin3 variants were associated with action potential prolongation. As Caveolin1 was recently identified in cardiomyocytes, we hypothesize that conserved isoform-specific protein interactions underlie human loss-of-function variants. To analyze the Caveolin1 and Caveolin3 interactome, we developed unbiased live-cell proteomic and isoform-specific mass spectrometry techniques. We demonstrate the functional relevance and pathogenic mechanism of a novel Caveolin3 interactor in gene-edited human iPSC-cardiomyocyte models.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart, Cardiac Muscle Cell

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Christof Lenz

LAB HEAD: Christof Lenz

PROVIDER: PXD019398 | Pride | 2022-10-30

REPOSITORIES: Pride

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			Action	DRS
	J_Peper_090617_010717_L2_R1_01.raw	Raw
	J_Peper_090617_010717_L2_R1_02.raw	Raw
	J_Peper_090617_010717_L2_R1_03.raw	Raw
	J_Peper_090617_010717_L2_R1_04.raw	Raw
	J_Peper_090617_010717_L2_R1_05.raw	Raw

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The Caveolin3 complex stabilizes functional surface expression of the Lactate-Proton Shuttle McT1 but not the sodium channel Nav1.5 in cardiomyocytes - Part 2: Ratiometric Proximity Proteomics

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