Dataset Information

Engineered chemotaxis core signaling units

ABSTRACT: Identificaiton of bacterial chemoreceptors by DSSO-based MS2-MS3 method.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Escherichia Coli

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Sheng Zhang

LAB HEAD: SHENG ZHANG

PROVIDER: PXD019599 | Pride | 2021-09-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	AliceMuok10377689_CheY_ADP-Try_ChymoTry_re.mgf	Mgf
	AliceMuok10377689_CheY_ADP_Try_ChymoTry_re.msf	Msf
	AliceMuok10377689_CheY_ADP_Try_ChymoTry_re.raw	Raw
	AlisaMuok10379162_TarFoComplex-DSSO2fileCombFinal.mgf	Mgf
	AlisaMuok10379162_TarFoComplex-DSSO2fileCombFinal.msf	Msf

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Publications

Engineered chemotaxis core signaling units indicate a constrained kinase-off state.

Muok Alise R AR Chua Teck Khiang TK Srivastava Madhur M Yang Wen W Maschmann Zach Z Borbat Petr P PP Chong Jenna J Zhang Sheng S Freed Jack H JH Briegel Ariane A Crane Brian R BR

Science signaling 20201110 657

Bacterial chemoreceptors, the histidine kinase CheA, and the coupling protein CheW form transmembrane molecular arrays with remarkable sensing properties. The receptors inhibit or stimulate CheA kinase activity depending on the presence of attractants or repellants, respectively. We engineered chemoreceptor cytoplasmic regions to assume a trimer of receptor dimers configuration that formed well-defined complexes with CheA and CheW and promoted a CheA kinase-off state. These mimics of core signal ...[more]

PMID: 33172954

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