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Quantitative LC-MS/MS Proteomics of human liver microsomes

ABSTRACT: We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in 20 human liver microsomal samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses for a wide variety of drugs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Jill Barber

LAB HEAD: Jill Barber

PROVIDER: PXD020910 | Pride | 2021-09-08

REPOSITORIES: Pride

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			Action	DRS
	Adultliversampleskeyanddemographic.docx	Other
	L01a.raw	Raw
	L01b.raw	Raw
	L02a.raw	Raw
	L02b.raw	Raw

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Publications

Mass spectrometry-based abundance atlas of ABC transporters in human liver, gut, kidney, brain and skin.

Al-Majdoub Zubida M ZM Achour Brahim B Couto Narciso N Howard Martyn M Elmorsi Yasmine Y Scotcher Daniel D Alrubia Sarah S El-Khateeb Eman E Vasilogianni Areti-Maria AM Alohali Noura N Neuhoff Sibylle S Schmitt Lutz L Rostami-Hodjegan Amin A Barber Jill J

FEBS letters 20201203 23

ABC transporters (ATP-binding cassette transporter) traffic drugs and their metabolites across membranes, making ABC transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low-abundance transmembrane proteins. Here, we analysed 200 samples of crude and membrane-enriched fractions from human liver, kidney, intestine, brain microvessels and skin, by ...[more]

PMID: 33128234

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2021-09-09 | PXD021018 | Pride

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2021-09-08 | PXD020742 | Pride

Quantitative LC-MS/MS proteomics of human kidney microsomes

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Quantitative LC-MS/MS Proteomics of paediatric human liver microsomes

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MicroRNA discovery and profiling in human embryonic stem cells by deep sequencing of small RNA libraries

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