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Quantitative LC-MS/MS Proteomics of human liver microsomes

ABSTRACT: We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in 20 human liver microsomal samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses for a wide variety of drugs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Jill Barber

LAB HEAD: Jill Barber

PROVIDER: PXD020910 | Pride | 2021-09-08

REPOSITORIES: Pride

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			Action	DRS
	Adultliversampleskeyanddemographic.docx	Other
	L01a.raw	Raw
	L01b.raw	Raw
	L02a.raw	Raw
	L02b.raw	Raw

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Publications

Mass spectrometry-based abundance atlas of ABC transporters in human liver, gut, kidney, brain and skin.

Al-Majdoub Zubida M ZM Achour Brahim B Couto Narciso N Howard Martyn M Elmorsi Yasmine Y Scotcher Daniel D Alrubia Sarah S El-Khateeb Eman E Vasilogianni Areti-Maria AM Alohali Noura N Neuhoff Sibylle S Schmitt Lutz L Rostami-Hodjegan Amin A Barber Jill J

FEBS letters 20201203 23

ABC transporters (ATP-binding cassette transporter) traffic drugs and their metabolites across membranes, making ABC transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low-abundance transmembrane proteins. Here, we analysed 200 samples of crude and membrane-enriched fractions from human liver, kidney, intestine, brain microvessels and skin, by ...[more]

PMID: 33128234

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Quantitative LC-MS/MS Proteomics of microsomal fractions from adult human livers

Project description:We report label-free and targeted quantification of xenobiotic metabolizing enzymes (XME) and transporters in 39 human liver microsomal samples. More than 2800 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models for dosage regimen design and precision dosing.

2021-09-09 | PXD021025 | Pride

Quantitative LC-MS/MS proteomics of human kidney microsomes

Project description:The aim of this study was to quantify enzymes and transporters in 20 human kidney cortex fractions. Using targeted accurate mass retention time (AMRT) and global proteomic approaches, 6 UGTs, 3 CYPs, 22 transporters, 1 adhesion and 1 plasma membrane marker were quantified; Eight of these proteins were identified for the first time in the kidney. The global proteomic method identified >4000 proteins. Kidney CYP2B6, CYP3A5 and CYP4F2 showed generally low expression.

2021-08-30 | PXD020996 | Pride

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2021-09-09 | PXD020987 | Pride

Quantitative LC-MS/MS Proteomics of human brain microvessels

Project description:We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases, nucleases and tight junction proteins in 22 human brain microvessel samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict drug disposition in the brain and permitting dose adjustment (precision dosing) in special populations of patients, such as those with dementia.

2021-09-09 | PXD021018 | Pride

Quantitative LC-MS/MS Proteomics of paediatric biliary atresia human liver microsomes

Project description:We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in 25 human liver microsomal samples, taken from patients with biliary atresia. Nearly 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses drugs used in biliary atresia patients.

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Quantitative LC-MS/MS Proteomics of paediatric human liver microsomes

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2021-09-09 | PXD020844 | Pride

Quantitative LC-MS/MS Proteomics of paediatric human liver microsomes (set 2)

Project description:We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in 24 human liver microsomal samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses for a wide variety of drugs.

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2021-09-08 | PXD020742 | Pride

Quantitative LC-MS/MS Proteomics of human liver microsomes (healthy controls and normal-tumour from cancer patients)

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2023-05-10 | PXD038776 | Pride

MicroRNA discovery and profiling in human embryonic stem cells by deep sequencing of small RNA libraries

Project description:We used massively parallel pyrosequencing to discover and characterize microRNAs (miRNAs) expressed in human embryonic stem cells (hESCs). Sequencing of small RNA cDNA libraries derived from undifferentiated hESC and from isogenic differentiating cultures yielded a total of 425,505 high-quality sequence reads. A custom data analysis pipeline delineated expression profiles for 191 previously annotated miRNAs, 36 novel miRNAs highly conserved across vertebrates, and 291 novel candidate miRNAs. A set of nine known and seven novel miRNAs were expressed in undifferentiated hESC and then strongly down-regulated with differentiation. Predicted mRNA targets of these miRNAs exhibited statistically significant enrichment for Gene Ontology functional categories relevant to ESC biology. Our study reveals that hESC express a larger complement of miRNAs than previously appreciated and it provides a resource for future studies of miRNA-mediated regulation in human embryonic stem cells. Two samples were 454 sequenced: undifferentiated human embryonic stem cells and differentiated human embryonic stem cells.

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