Dataset Information

Quantitative LC-MS/MS Proteomics of human small intestine

ABSTRACT: The objective was to determine the expression of a wide range proteins pertinent to metabolism and disposition of chemicals and nutrients in the intestinal epithelium. Ileum and jejunum biopsy specimens were obtained from 16 patients undergoing gastrointestinal elective surgery. Mucosal fractions were prepared and analysed using targeted and global proteomic approaches. A total of 29 enzymes, 32 transporters, 6 tight junction proteins, 2 adhesion proteins, alkaline phosphatase, thioredoxin, 5 markers and 1 regulatory protein were quantified; 60 for the first time.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Small Intestine Mucosa

SUBMITTER: Jill Barber

LAB HEAD: Jill Barber

PROVIDER: PXD020987 | Pride | 2021-09-09

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20180717_BarberJ_ZA01_batch2_180min.raw	Raw
	20180717_BarberJ_ZA10_batch2_180min.raw	Raw
	20180717_BarberJ_ZA20_batch2_180min.raw	Raw
	20180727_BarberJ_ZA01a.raw	Raw
	20180727_BarberJ_ZA05a.raw	Raw

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Publications

Mass spectrometry-based abundance atlas of ABC transporters in human liver, gut, kidney, brain and skin.

Al-Majdoub Zubida M ZM Achour Brahim B Couto Narciso N Howard Martyn M Elmorsi Yasmine Y Scotcher Daniel D Alrubia Sarah S El-Khateeb Eman E Vasilogianni Areti-Maria AM Alohali Noura N Neuhoff Sibylle S Schmitt Lutz L Rostami-Hodjegan Amin A Barber Jill J

FEBS letters 20201203 23

ABC transporters (ATP-binding cassette transporter) traffic drugs and their metabolites across membranes, making ABC transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low-abundance transmembrane proteins. Here, we analysed 200 samples of crude and membrane-enriched fractions from human liver, kidney, intestine, brain microvessels and skin, by ...[more]

PMID: 33128234

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