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Quantitative LC-MS/MS Proteomics of microsomal fractions from adult human livers

ABSTRACT: We report label-free and targeted quantification of xenobiotic metabolizing enzymes (XME) and transporters in 39 human liver microsomal samples. More than 2800 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models for dosage regimen design and precision dosing.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Liver

SUBMITTER: Brahim Achour

LAB HEAD: Jill Barber

PROVIDER: PXD021025 | Pride | 2021-09-09

REPOSITORIES: Pride

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	20160302_ZA_01.raw	Raw
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Publications

Mass spectrometry-based abundance atlas of ABC transporters in human liver, gut, kidney, brain and skin.

Al-Majdoub Zubida M ZM Achour Brahim B Couto Narciso N Howard Martyn M Elmorsi Yasmine Y Scotcher Daniel D Alrubia Sarah S El-Khateeb Eman E Vasilogianni Areti-Maria AM Alohali Noura N Neuhoff Sibylle S Schmitt Lutz L Rostami-Hodjegan Amin A Barber Jill J

FEBS letters 20201203 23

ABC transporters (ATP-binding cassette transporter) traffic drugs and their metabolites across membranes, making ABC transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains challenging because they are large and low-abundance transmembrane proteins. Here, we analysed 200 samples of crude and membrane-enriched fractions from human liver, kidney, intestine, brain microvessels and skin, by ...[more]

PMID: 33128234

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Quantitative LC-MS/MS Proteomics of human liver microsomes

Project description:We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in 20 human liver microsomal samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses for a wide variety of drugs.

2021-09-08 | PXD020910 | Pride

Quantitative LC-MS/MS Proteomics of paediatric biliary atresia human liver microsomes

Project description:We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in 25 human liver microsomal samples, taken from patients with biliary atresia. Nearly 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses drugs used in biliary atresia patients.

2021-09-09 | PXD020974 | Pride

Quantitative LC-MS/MS Proteomics of paediatric human liver microsomes

2021-09-09 | PXD020844 | Pride

Quantitative LC-MS/MS Proteomics of paediatric human liver microsomes (set 2)

Project description:We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in 24 human liver microsomal samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses for a wide variety of drugs.

2021-09-09 | PXD020939 | Pride

Quantitative LC-MS/MS Proteomics of human small intestine

Project description:The objective was to determine the expression of a wide range proteins pertinent to metabolism and disposition of chemicals and nutrients in the intestinal epithelium. Ileum and jejunum biopsy specimens were obtained from 16 patients undergoing gastrointestinal elective surgery. Mucosal fractions were prepared and analysed using targeted and global proteomic approaches. A total of 29 enzymes, 32 transporters, 6 tight junction proteins, 2 adhesion proteins, alkaline phosphatase, thioredoxin, 5 markers and 1 regulatory protein were quantified; 60 for the first time.

2021-09-09 | PXD020987 | Pride

Quantitative LC-MS/MS Proteomics of human liver microsomes (healthy controls and normal-tumour from cancer patients)

Project description:We report quantification of proteins in human liver microsomal samples from 15 healthy volunteers and 18 patients with cancer in the liver (mainly, colorectal cancer liver metastasis). These data can be used in physiologically based pharmacokinetic models to predict appropriate drug doses in patients with cancer in their liver, especially colorectal cancer liver metastasis.

2023-05-10 | PXD038776 | Pride

Quantitative LC-MS/MS proteomics of human kidney microsomes

Project description:The aim of this study was to quantify enzymes and transporters in 20 human kidney cortex fractions. Using targeted accurate mass retention time (AMRT) and global proteomic approaches, 6 UGTs, 3 CYPs, 22 transporters, 1 adhesion and 1 plasma membrane marker were quantified; Eight of these proteins were identified for the first time in the kidney. The global proteomic method identified >4000 proteins. Kidney CYP2B6, CYP3A5 and CYP4F2 showed generally low expression.

2021-08-30 | PXD020996 | Pride

Quantitative LC-MS/MS Proteomics of human brain microvessels

Project description:We report label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases, nucleases and tight junction proteins in 22 human brain microvessel samples. More than 3500 proteins were identified and quantified. These data can be used in physiologically based pharmacokinetic models to predict drug disposition in the brain and permitting dose adjustment (precision dosing) in special populations of patients, such as those with dementia.

2021-09-09 | PXD021018 | Pride

Quantitative LC-MS/MS Proteomics of human skin and LabSkin model

Project description:We report for the first time label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in six human skin explants and a 3D living skin equivalent model from LabSkin. More than 2000 proteins were identified and quantified from total cellular protein. This systematic determination of functional equivalence between human skin and LabSkin is a key step towards the construction of a representative human in vitro skin model, which can be used as an alternative to current animal-based tests for chemical safety and for predicting dosage of topically administered drugs.

2021-09-08 | PXD020742 | Pride

Single cell RNA-sequencing of E8.5 stage embryonic neural tissue from mouse

Project description:We describe a so far uncharacterized, embryonic and self-renewing Neural Plate Border Stem Cell (NBSC) population with the capacity to differentiate into central nervous and neural crest lineages. NBSCs can be obtained by neural transcription factor-mediated reprogramming (BRN2, SOX2, KLF4, and ZIC3) of human adult dermal fibroblasts and peripheral blood cells (induced Neural Plate Border Stem Cells, iNBSCs) or by directed differentiation from human induced pluripotent stem cells (NBSCs). Moreover, human (i)NBSCs share molecular and functional features with primary Neural Plate Border Stem Cells (pNBSCs) isolated from neural folds of E8.5 mouse embryos. Here we provide single cell RNA-sequencing data of neural tissue derived from two E8.5 mouse embryos. After manual isolation and enzymatic separation E8.5 neural tissue was single cell sorted and RNA sequencing was performed following the Smart-seq2 protocol. In sum, cultured pNBSCs and E8.5 neural tube cells share a similar regional identity and expression signature suggesting that pNBSCs might correspond to an endogenous progenitor in this area of the developing brain.

2018-12-20 | E-MTAB-6912 | biostudies-arrayexpress

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