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ITRAQ-based Quantitative Proteomics Analysis Screen for Critical Proteins during Temperature-Responsive Flowering of Crocus sativus

ABSTRACT: To uncover the underlying mechanism of temperature-responsive flowering in Crocus sativus and unveil flowering regulatory proteins, iTRAQ (isobaric tags for relative or absolute quantitation)-based proteomics were applied to study the differences in protein expression profiles in flowering and non-flowering saffron grown at room- and low-temperatures, respectively. A total of 5,624 proteins were identified，and 201 proteins showed differential accumulations between the flowering and non-flowering groups.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Crocus Sativus

TISSUE(S): Plant Bud

SUBMITTER: Liqin Li

LAB HEAD: Liqin Li

PROVIDER: PXD021020 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

Screening of Key Proteins Affecting Floral Initiation of Saffron Under Cold Stress Using iTRAQ-Based Proteomics.

Chen Jing J Zhou Guifen G Dong Yan Y Qian Xiaodong X Li Jing J Xu Xuting X Huang Huilian H Xu Limin L Li Liqin L

Frontiers in plant science 20210511

<h4>Background</h4>Saffron crocus (<i>Crocus sativus</i>) is an expensive and valuable species that presents preventive and curative effects. This study aimed to screen the key proteins affecting the floral initiation of saffron under cold stress and thus increasing yield by regulating the temperature.<h4>Results</h4>Protein expression profiles in flowering and non-flowering saffron buds were established using isobaric tags for relative or absolute quantitation (iTRAQ). A total of 5,624 proteins ...[more]

PMID: 34046047

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