Dataset Information

Investigation of changes in the proteome and the phosphoproteome of the heart induced by exercise, either in control or in PKP2-deficient hearts in relation to ARVC

ABSTRACT: We performed an unbiased investigation of changes in the proteome and the phosphoproteome of the heart induced by exercise, either in control or in PKP2-deficient hearts. Two pairs of experimental groups were studied: The first pair consisted of control animals and the second pair of animals deficient in PKP2 (PKP2cKO). For both cases, results obtained from sedentary animals were compared to those obtained from animals subjected to six weeks of a running exercise program. We therefore refer to the first pair as “control” and the second pair as “PKP2cKO” and in both cases, the variable was “exercise”. Left ventricular tissue from these animals were subjected to quantitative proteome and phosphoproteome measurements applying tandem mass tag (TMT) labeling and high-resolution mass spectrometry measurements performed on an Orbitrap QExactive mass spectrometer. For phosphoproteomic analyses, peptides were enriched by titanium dioxide chromatography and all peptide samples were pre-fractionated into 12 fractions prior to measurement.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

DISEASE(S): Arrhythmogenic Right Ventricular Cardiomyopathy

SUBMITTER: Navratan Bagwan

LAB HEAD: Alicia Lundby

PROVIDER: PXD025956 | Pride | 2022-11-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20191223_QE9_nLC13_SM_SA_MOUSE_PKP2KO_LV_TMT_set1_frac_01.raw	Raw
	20191223_QE9_nLC13_SM_SA_MOUSE_PKP2KO_LV_TMT_set1_frac_02.raw	Raw
	20191223_QE9_nLC13_SM_SA_MOUSE_PKP2KO_LV_TMT_set1_frac_03.raw	Raw
	20191223_QE9_nLC13_SM_SA_MOUSE_PKP2KO_LV_TMT_set1_frac_04.raw	Raw
	20191223_QE9_nLC13_SM_SA_MOUSE_PKP2KO_LV_TMT_set1_frac_05.raw	Raw

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Publications

Exercise Causes Arrhythmogenic Remodeling of Intracellular Calcium Dynamics in Plakophilin-2-Deficient Hearts.

van Opbergen Chantal J M CJM Bagwan Navratan N Maurya Svetlana R SR Kim Joon-Chul JC Smith Abigail N AN Blackwell Daniel J DJ Johnston Jeffrey N JN Knollmann Björn C BC Cerrone Marina M Lundby Alicia A Delmar Mario M

Circulation 20220501 19

<h4>Background</h4>Exercise training, and catecholaminergic stimulation, increase the incidence of arrhythmic events in patients affected with arrhythmogenic right ventricular cardiomyopathy correlated with plakophilin-2 (PKP2) mutations. Separate data show that reduced abundance of PKP2 leads to dysregulation of intracellular Ca2+ (Ca2+i) homeostasis. Here, we study the relation between excercise, catecholaminergic stimulation, Ca2+i homeos ...[more]

PMID: 35491884

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