Dataset Information

First identification of Cr-binding proteome in human HepG2 cells

ABSTRACT: By integrating fluorescence imaging with proteomic approach, we visualized Cr3+ protein targets mainly localized in mitochondria and identified eight Cr3+-binding proteins, including ATP synthase catalytic subunit beta (ATP5B).

INSTRUMENT(S): 4800 Proteomics Analyzer

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Haibo Wang

LAB HEAD: Prof. Hongzhe Sun

PROVIDER: PXD027305 | Pride | 2023-05-10

REPOSITORIES: pride

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Publications

Mitochondrial ATP synthase as a direct molecular target of chromium(III) to ameliorate hyperglycaemia stress.

Wang Haibo H Hu Ligang L Li Hongyan H Lai Yau-Tsz YT Wei Xueying X Xu Xiaohan X Cao Zhenkun Z Cao Huiming H Wan Qianya Q Chang Yuen-Yan YY Xu Aimin A Zhou Qunfang Q Jiang Guibin G He Ming-Liang ML Sun Hongzhe H

Nature communications 20230328 1

Chromium(III) is extensively used as a supplement for muscle development and the treatment of diabetes mellitus. However, its mode of action, essentiality, and physiological/pharmacological effects have been a subject of scientific debate for over half a century owing to the failure in identifying the molecular targets of Cr(III). Herein, by integrating fluorescence imaging with a proteomic approach, we visualized the Cr(III) proteome being mainly localized in the mitochondria, and subsequently ...[more]

PMID: 36977671

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			Action	DRS
	J1_MS.mgf	Mgf
	J1_MS_1.t2d	Other
	J2_MS.mgf	Mgf
	J2_MS_1.t2d	Other
	J3_MS.mgf	Mgf