Dataset Information

Proteomic profiling of glioblastoma stem cells - HGCC cohort

ABSTRACT: With HiRIEF LC-MS/MS, we analysed 11 GBM stem cell lines from the HGCC cohort (Uppsala, Sweden). The samples were run in one TMT16 set, with three samples analysed in duplicates, and the last two channels left empty.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Malignant Cell, Glioblastoma Multiforme Cancer Stem Cell

DISEASE(S): Glioblastoma,Brain Cancer,Idh-wildtype Glioblastoma

SUBMITTER: Haris Babačić

LAB HEAD: Janne Lehtiö

PROVIDER: PXD027339 | Pride | 2022-12-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20200624_HGCC_GBM_stem_cell_lines_TMT16_HiRIEF_IPG3-10_fr02.raw	Raw
	20200624_HGCC_GBM_stem_cell_lines_TMT16_HiRIEF_IPG3-10_fr03.raw	Raw
	20200624_HGCC_GBM_stem_cell_lines_TMT16_HiRIEF_IPG3-10_fr04.raw	Raw
	20200624_HGCC_GBM_stem_cell_lines_TMT16_HiRIEF_IPG3-10_fr05.raw	Raw
	20200624_HGCC_GBM_stem_cell_lines_TMT16_HiRIEF_IPG3-10_fr06.raw	Raw

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Publications

Glioblastoma stem cells express non-canonical proteins and exclusive mesenchymal-like or non-mesenchymal-like protein signatures.

Babačić Haris H Galardi Silvia S Umer Husen M HM Hellström Mats M Uhrbom Lene L Maturi Nagaprathyusha N Cardinali Deborah D Pellegatta Serena S Michienzi Alessandro A Trevisi Gianluca G Mangiola Annunziato A Lehtiö Janne J Ciafrè Silvia Anna SA Pernemalm Maria M

Molecular oncology 20230116 2

Glioblastoma (GBM) cancer stem cells (GSCs) contribute to GBM's origin, recurrence, and resistance to treatment. However, the understanding of how mRNA expression patterns of GBM subtypes are reflected at global proteome level in GSCs is limited. To characterize protein expression in GSCs, we performed in-depth proteogenomic analysis of patient-derived GSCs by RNA-sequencing and mass-spectrometry. We quantified > 10 000 proteins in two independent GSC panels and propose a GSC-associated proteomi ...[more]

PMID: 36495079

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